TRANSFER OF A FOREIGN GENE INTO THE BRAIN USING ADENOVIRUS VECTORS
TRANSFER OF A FOREIGN GENE INTO THE BRAIN USING ADENOVIRUS VECTORS
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DOI：
10.1038/ng0393-224
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发表时间：
1993-03-01
期刊：
影响因子：
30.8
通讯作者：
PESCHANSKI, MR
中科院分区：
文献类型：
--
作者：
AKLI, S;CAILLAUD, C;PESCHANSKI, MR
分析了复制缺陷型腺病毒载体在体内将外源基因转移到成年大鼠神经细胞中的能力。大量的神经细胞（包括神经元，星形胶质细胞和室管膜细胞）表达大肠杆菌lacZ转基因至少45天后接种的各种脑区。在10穆尔中注射高达3 × 10（5）pfu不会导致任何可检测的细胞病变效应-这些仅在非常高滴度的感染（>10（7）pfu 10穆尔-1）中观察到。因此，腺病毒载体似乎是一个有前途的手段，在体内转移治疗基因进入中枢神经系统。
The ability of a replication-deficient adenovirus vector to transfer a foreign gene into neural cells of adult rats in vivo has been analysed. A large number of neural cells (including neurons, astrocytes and ependymal cells) expressed an E coli lacZ transgene for at least 45 days after inoculation of various brain areas. Injecting up to 3 x 10(5) pfu in 10 mul did not result in any detectable cytopathic effects - these were only observed for very high titres of infection (>10(7) pfu 10 mul-1). Adenovirus vectors therefore appear to be a promising means for in vivo transfer of therapeutic genes into the central nervous system.