Development of bacteria-based microrobot using biocompatible poly(ethylene glycol)
Development of bacteria-based microrobot using biocompatible poly(ethylene glycol)
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DOI：
10.1007/s10544-012-9704-1
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Park, Sukho
中科院分区：
文献类型：
--
作者：
Cho, Sunghoon;Park, Sung Jun;Park, Sukho
为了研制基于细菌的生物医学微机器人，我们提出了一种利用交叉连接微流通道制备生物相容性聚乙二醇（PEG）微球的方法。通过紫外线（UV）照射使PEG液滴聚合以在微流体通道中形成直径为8.18 +/-3.4 μ m的PEG微珠。一般来说，由于PEG微珠的亲水性，细菌不会附着在PEG微珠的表面。我们使用聚-L-赖氨酸（PLL）修饰PEG微珠的选择性表面，使用PEG微珠在琼脂糖凝胶上的浸没特性促进减毒鼠伤寒沙门氏菌粘附：细菌因此可以附着到PEG微珠的PLL涂覆的表面区域。选择性PLL包被的PEG微珠组显示出增强的运动性与PLL未包被和完全PLL包被的PEG微珠组相比。选择性PLL包被的PEG微珠组显示出比PLL未包被和完全PLL包被的PEG微珠组分别高12.33和7.40倍的平均速度。本研究验证了使用PEG微珠的基于细菌的微型机器人的成功制造，以及通过使用琼脂糖凝胶和PLL的选择性细菌图案化来增强微型机器人的运动性。
For the development of bacteria-based biomedical microrobot, we propose the fabrication method of biocompatible poly(ethylene glycol) (PEG) microbeads using a cross-junction microfluidic channel. PEG droplets were polymerized by ultraviolet (UV) irradiation to form PEG microbeads of 8.18 +/- 3.4 mu m diameter in a microfluidic channel. Generally, the bacteria did not attach to the surface of the PEG microbeads because of their hydrophilicity. We modified the selective surface of the PEG microbeads using poly-L-lysine (PLL), promoting attenuated Salmonella typhimurium adhesion using the submerging property of PEG microbeads on agarose gel: the bacteria could thus be attached to the PLL-coated surface region of the PEG microbeads. The selectively PLL-coated PEG microbeads group showed enhanced motility compared with the PLL-uncoated and completely PLL-coated PEG microbeads groups. The selectively PLL-coated PEG microbeads group showed 12.33 and 7.40 times higher average velocities than the PLL-uncoated and completely PLL-coated PEG microbeads groups, respectively. This study verified the successful fabrication of bacteria-based microrobots using PEG microbeads, and the enhanced motility of the microrobots by selective bacteria patterning using agarose gel and PLL.