Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry
Simultaneous determination of Ziagen and its phosphorylated metabolites by ion-pairing high-performance liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/s0378-4347(00)00619-8
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发表时间：
2001-04-25
期刊：
影响因子：
--
通讯作者：
Sinhababu, AK
中科院分区：
文献类型：
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作者：
Fung, EN;Cai, ZW;Sinhababu, AK
建立了一种同时定量齐阿根、单磷酸卡波韦、二磷酸卡波韦和三磷酸卡波韦的正离子模式电喷雾离子对HPLC-MS-MS方法。N ′，N ′-二甲基己胺用作离子对试剂。这种离子配对剂的存在下，允许保留和分离的四种化合物的反相HPLC柱，以及检测的核苷酸与正离子模式电喷雾电离。发现所有分析物的检测限均优于25 nM。分析物的校准曲线在25 nM至5 μ M的范围内显示出优异的线性。质控样品重复分析的相对标准偏差和准确度均小于15%。该方法已成功地应用于分析这些化合物与Ziagen处理的人肝细胞。(C)2001 Elsevier Science B. V.保留所有权利。
An ion-paring HPLC-MS-MS method with positive ion mode electrospray ionization has been developed to simultaneously quantify Ziagen, carbovir monophosphate, carbovir diphosphate and carbovir triphosphate. N',N'-Dimethylhexylamine was used as the ion-pairing agent. The presence of this ion-pairing agent allowed the retention and separation of the four compounds on a reversed-phase HPLC column as well as the detection of the nucleotides with positive ion mode electrospray ionization. The limits of detection were found to be better than 25 nM for all the analytes. Calibration curves of the analytes showed excellent linearity over the range of 25 nM to 5 muM. The relative standard deviations and accuracies for replicate analyses of quality control samples were less than 15%. The method has been successfully applied to the analysis of these compounds in human liver cells treated with Ziagen. (C) 2001 Elsevier Science B.V. All rights reserved.