The prediction and characterization of YshA, an unknown outer-membrane protein from Salmonella typhimurium.
The prediction and characterization of YshA, an unknown outer-membrane protein from Salmonella typhimurium.
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DOI：
10.1016/j.bbamem.2010.09.008
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Wimley WC
中科院分区：
文献类型：
--
作者：
Freeman TC Jr;Landry SJ;Wimley WC
We have developed an effective pathway for the prediction and characterization of novel transmembrane β-barrel proteins. The Freeman-Wimley algorithm, which is a highly accurate prediction method based on the physicochemical properties of experimentally characterized transmembrane β barrel (TMBB) structures, was used to predict TMBBs in the genome of Salmonella typhimurium LT2. The previously uncharacterized product of gene yshA was tested as a model for validating the algorithm. YshA is a highly conserved 230-residue protein that is predicted to have 10 transmembrane β-strands and an N-terminal signal sequence. All of the physicochemical and spectroscopic properties exhibited by YshA are consistent with the prediction that it is a TMBB. Specifically, recombinant YshA localizes to the outer membrane when expressed in Escherichia coli; YshA has β-sheet-rich secondary structure with stable tertiary contacts in the presence of detergent micelles or when reconstituted into a lipid bilayer; when in a lipid bilayer, YshA forms a membrane-spanning pore with an effective radius of ~0.7 nm. Taken together, these data substantiate the predictions made by the Freeman-Wimley algorithm by showing that YshA is a TMBB protein.
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影响因子：
8
作者：
Kleinschmidt, JH;Wiener, MC;Tamm, LK
通讯作者：
Tamm, LK
影响因子：
3.4
作者：
Bay, Denice C.;O'Neil, Joe D.;Court, Deborah A.
通讯作者：
Court, Deborah A.
DOI：
10.1073/pnas.0400358101
发表时间：
2004-03-23
影响因子：
11.1
作者：
Hong, HD;Tamm, LK
通讯作者：
Tamm, LK
影响因子：
1.6
作者：
Charbonnier, F;Köhler, T;Ducruix, A
通讯作者：
Ducruix, A
DOI：
10.1038/nsb914
发表时间：
2003-05-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Chimento, DP;Mohanty, AK;Wiener, MC
通讯作者：
Wiener, MC