Construction of human-mouse T cell hybrids that express human T cell-associated surface antigens and allow the chromosomal localization of these antigens.
Construction of human-mouse T cell hybrids that express human T cell-associated surface antigens and allow the chromosomal localization of these antigens.
复制标题
构建人-小鼠 T 细胞杂交体，表达人 T 细胞相关表面抗原并允许这些抗原的染色体定位。
作者：
J. E. V. Dongen;A. Kessel;I. Wolvers;M. Versnel;A. Oudenaren;E. Schoenmaker;A. Hagemeijer
我们已经构建了小鼠T细胞系BW 5147和T细胞急性淋巴细胞白血病患者的细胞之间的体细胞杂交。分析获得的杂交克隆的人T细胞抗原的表达和人染色体的存在。由BW 5147细胞系和来自前T急性淋巴细胞白血病患者的骨髓细胞（TdT+/HLA-DR+/Tp 41 +/T11+/T1-/T6-/T4-/T8-/T3-）之间的融合衍生的T细胞杂交体似乎表达人T细胞抗原Tp 41，其可以被单克隆抗体3A 1和WT 1识别。虽然这组杂交细胞含有所有人类染色体，但没有其他T细胞抗原表达。BW 5147细胞系与来自患有更成熟的T细胞急性淋巴细胞白血病（TdT+/HLA-DR+/Tp 41 +/T11+/T1+/T6-/T4+/T8+/T3-）的患者的外周血细胞的融合产生了一组杂交克隆，其不仅表达Tp 41抗原，而且表达人T细胞抗原T1和T4;两个杂交体甚至表达T3抗原。这组混血儿也包含了整个人类基因组。两组人-小鼠T细胞杂交体使我们能够将编码人类T细胞抗原Tp 41、T1和T4的基因分别分配到人类17、11和12号染色体。此外，这些数据支持我们以前的建议，即人淋巴分化抗原在人-小鼠淋巴杂交的表达是由融合伙伴的分化阶段的影响。
We have constructed somatic cell hybrids between the murine T cell line BW5147 and cells from patients suffering from T cell acute lymphoblastic leukemia. The obtained hybrid clones were analyzed for expression of human T cell antigens and presence of human chromosomes. T cell hybrids derived from fusion between the BW5147 cell line and bone marrow cells from a patient with pre-T acute lymphoblastic leukemia (TdT+/HLA-DR+/Tp41+/T11+/T1-/T6-/T4-/T8-/T3-) appeared to express the human T cell antigen Tp41, which can be recognized by the monoclonal antibodies 3A1 and WT1. Although this panel of hybrid cells contained all human chromosomes, no other T cell antigens were expressed. Fusion of the BW5147 cell line with peripheral blood cells from a patient with a more mature T cell acute lymphoblastic leukemia (TdT+/HLA-DR+/Tp41+/T11+/T1+/T6-/T4+/T8+/T3-) resulted in a panel of hybrid clones that expressed not only the Tp41 antigen, but also the human T cell antigens T1 and T4; two hybrids even expressed the T3 antigen. This panel of hybrids also contained the whole human genome. The two panels of human-mouse T cell hybrids allowed us to assign the genes coding for the human T cell antigens Tp41, T1, and T4 to human chromosomes 17, 11, and 12, respectively. Furthermore, these data support our previous suggestion that the expression of human lymphoid differentiation antigens in human-mouse lymphoid hybrids is influenced by the differentiation stage of the fusion partners.