Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo
Cholinergic modulation of spindle bursts in the neonatal rat visual cortex in vivo
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DOI：
10.1523/jneurosci.5233-06.2007
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发表时间：
2007-05-23
影响因子：
5.3
通讯作者：
Khazipov, Rustem
中科院分区：
文献类型：
--
作者：
Hanganu, Ileana L.;Staiger, Jochen F.;Khazipov, Rustem
乙酰胆碱（ACh）是已知的形状成人的新皮层活动有关的行为状态和处理的感觉信息。然而，胆碱能输入对新生儿神经元活动的影响仍然广泛未知。在发育早期，初级视觉（V1）皮层的主要活动模式是间歇性的自组织纺锤波爆发振荡，可以由视网膜波驱动。在这里，我们评估了这种早期活动模式和胆碱能驱动器之间的关系，通过阻断或增强胆碱能输入，并调查在出生后第一周内在体内对大鼠视皮层活动的影响。通过脑室内、皮质内或皮质上应用阿托品阻断毒蕈碱受体，可使V1纺锤波爆发的发生率降低50%，视网膜非依赖性和视神经介导的纺锤波爆发均受到影响。相反，阻断乙酰胆碱酯酶与毒扁豆碱增强的发生，振幅和持续时间的V1纺锤波爆发。而直接刺激胆碱能基底前脑核增加了V1纺锤体爆发的发生概率，其慢性免疫毒性病变使用192 IgG-皂草素降低新生儿V1振荡活动的发生率为87%。因此，胆碱能输入促进新生儿V1纺锤波爆发，并可能引发发育中的皮层专门操作相关的早期（视网膜波）和后期（视觉输入）刺激。
Acetylcholine ( ACh) is known to shape the adult neocortical activity related to behavioral states and processing of sensory information. However, the impact of cholinergic input on the neonatal neuronal activity remains widely unknown. Early during development, the principal activity pattern in the primary visual ( V1) cortex is the intermittent self-organized spindle burst oscillation that can be driven by the retinal waves. Here, we assessed the relationship between this early activity pattern and the cholinergic drive by either blocking or augmenting the cholinergic input and investigating the resultant effects on the activity of the rat visual cortex during the first postnatal week in vivo. Blockade of the muscarinic receptors by intracerebroventricular, intracortical, or supracortical atropine application decreased the occurrence of V1 spindle bursts by 50%, both the retina-independent and the optic nerve-mediated spindle bursts being affected. In contrast, blockade of acetylcholine esterase with physostigmine augmented the occurrence, amplitude, and duration of V1 spindle bursts. Whereas direct stimulation of the cholinergic basal forebrain nuclei increased the occurrence probability of V1 spindle bursts, their chronic immunotoxic lesion using 192 IgG-saporin decreased the occurrence of neonatal V1 oscillatory activity by 87%. Thus, the cholinergic input facilitates the neonatal V1 spindle bursts and may prime the developing cortex to operate specifically on relevant early ( retinal waves) and later ( visual input) stimuli.