Swarming Motility Assays in Salmonella.
Swarming Motility Assays in Salmonella.
复制标题
沙门氏菌的群体运动测定。
DOI：
10.1007/978-1-0716-3060-0_13
复制
发表时间：
2023
期刊：
影响因子：
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通讯作者：
Harshey,RasikaM
中科院分区：
文献类型：
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作者：
Partridge,JonathanD;Harshey,RasikaM
肠道沙门氏菌有6个亚种，其中肠道亚种对人类健康最为重要。该物种的传播和传染性依赖于鞭毛驱动的运动。鞭毛介导的两种运动已经被描述过：在散装液体中单独游动和在表面基质上集体游动。在蜂群中，细菌获得了独特的生理机能，最重要的结果是获得了对抗生素的适应性抗性。这里描述了培养、验证和研究游泳和群体运动的协议。另外还有一项“跨界”试验，在该试验中，“准备”蜂群的细胞面临抗生素等环境挑战，以评估它们应对挑战的倾向。
Salmonella entericahas six subspecies, of which the subspeciesentericais the most important for human health. The dispersal and infectivity of this species are dependent upon flagella-driven motility. Two kinds of flagella-mediated movements have been described—swimming individually in bulk liquid and swarming collectively over a surface substrate. During swarming, the bacteria acquire a distinct physiology, the most significant consequence of which is acquisition of adaptive resistance to antibiotics. Described here are protocols to cultivate, verify, and study swimming and swarming motility inS. enterica, and an additional “border-crossing” assay, where cells “primed” to swarm are presented with an environmental challenge such as antibiotics to assess their propensity to handle the challenge.