Luteinizing hormone-releasing hormone neuronal system during the estrous cycle of the female rat: effects of surgically induced persistent estrus.
Luteinizing hormone-releasing hormone neuronal system during the estrous cycle of the female rat: effects of surgically induced persistent estrus.
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雌性大鼠发情周期期间黄体生成素释放激素神经元系统：手术诱导持续发情的影响。
DOI：
10.1159/000124583
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发表时间：
1986
影响因子：
4.1
通讯作者：
Kelly,MJ
中科院分区：
文献类型：
--
作者：
Ronnekleiv,OK;Kelly,MJ
研究了视交叉上核和内侧视前核离散损伤对雌性大鼠黄体生成素释放激素（LHRH）神经元的影响。这些病变扰乱了动情周期，并阻止了促黄体生成素和催乳素的排卵前激增。损伤后两到三个月，对对照动物和损伤动物进行灌注，将脑切片，并使用过氧化物酶抗过氧化物酶方法和高滴度 LHRH 构象抗血清对组织进行 LHRH 免疫细胞化学处理。在发情周期的所有阶段，在视前区和基底下丘脑中均观察到染色微弱的 LHRH 细胞。免疫反应细胞体的数量从发情前期的 583 个细胞的最高值到发情期的 35 个细胞的最低值（平均值 ± SEM = 323 ± 59；n = 11）。相反，有病变的持续发情动物表现出病变头侧、侧向和尾侧的 LHRH 神经元强度和数量增加。每只动物的细胞总数为 625 至 954 个细胞（平均值 ± SEM = 784 ± 44；n = 8；与对照相比，p< 0.001）。此外，所有受损动物在正中隆起区域都表现出强烈的纤维染色。总之，这些数据支持这样的假设：持续发情是由直接或间接控制 LHRH 神经元的神经元破坏引起的。
The effects of discrete lesions of the suprachiasmatic and medial preoptic nucleus on luteinizing hormone-releasing hormone (LHRH) neurons of the female rat were examined. The lesions disrupted the estrous cycle and prevented the preovulatory surge of luteinizing hormone and prolactin. Two to three months following the lesions, control and lesioned animals were perfused, the brains were sectioned, and the tissue processed for LHRH immunocytochemistry using the peroxidase antiperoxidase method and a high-titer, conformational antiserum to LHRH. Faintly stained LHRH cells were observed in the preoptic area and the basal hypothalamus at all stages of the estrous cycle. The number of immunoreactive cell bodies varied from a high of 583 cells on proestrus, to a low of 35 cells on estrus (mean ± SEM = 323 ± 59; n = 11). In contrast, the constant estrous animals with lesions showed increased intensity and number of LHRH neurons rostral, lateral and caudal to the lesion. The total number of cells ranged from 625 to 954 cells per animal (mean ± SEM = 784 ± 44; n = 8; p< 0.001 vs. controls). Moreover, all lesioned animals exhibited intense fiber stain in the median eminence region. In conclusion, these data support the hypothesis that persistent estrus is caused by destruction of neurons which directly or indirectly control LHRH neurons.