The immunologic response to infection with respiratory syncytial virus in infants.
The immunologic response to infection with respiratory syncytial virus in infants.
复制标题
婴儿呼吸道合胞病毒感染的免疫反应。
DOI：
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发表时间：
1978
影响因子：
6.4
通讯作者：
R. Barkin
中科院分区：
文献类型：
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作者：
K. Mcintosh;H. Masters;I. Orr;R. Chao;R. Barkin
对50名6个月以下因呼吸道合胞病毒(RSV)感染住院的婴儿进行了鼻分泌物系列样本的检测。分泌中和活性用斑块减少法测定，分泌抗体用人免疫球蛋白A、免疫球蛋白G或免疫球蛋白M偶联抗血清间接荧光法测定。感染期间分泌中和活性上升或下降四倍，频率大致相同(分别为20%和26%)。相比之下，分泌物中RSV的IgA抗体水平上升了四倍，56%-65%的婴儿中没有下降。抗体滴度升高的频率与年龄直接相关。在个体分泌物中，中和活性与RSV的IgA抗体之间的相关性很差：在没有可检测到的抗体的情况下，经常发现中和活性，而抗RSV的IgA抗体往往是非中和的。然而，呼吸道合胞病毒IgA抗体的产生与病毒从呼吸道中消失的时间相关。这种分泌反应的时机与抗体对治愈感染有显著贡献的假设是一致的。
Fifty infants younger than six months, hospitalized for infection with respiratory syncytial virus (RSV), were studied by examination of serial samples of nasal secretion. Secretory neutralizing activity was measured by plaque reduction and secretory antibody by indirect fluorescence using conjugated antiserum to human IgA, IgG, or IgM. Secretory neutralizing activity during infection rose or fell fourfold with approximately equal frequency (20% and 26%, respectively). In contrast, levels of IgA antibody to RSV in secretions rose fourfold in 56%--65% of the infants and fell in none. The frequency of such rises in titer of antibody was directly related to age. In individual secretions the correlation between neutralizing activity and IgA antibody to RSV was poor: neutralizing activity was often found in the absence of detectable antibody, and IgA antibody to RSV was often nonneutralizing. Nevertheless, the development of IgA antibody to RSV correlated in time with the disappearance of virus from the respiratory tract. The timing of this secretory response is consistent with the hypothesis that antibody contributes significantly to cure of infection.