The intracellular immune receptor like gene SNC1 is an enhancer of effector-triggered immunity in Arabidopsis
The intracellular immune receptor like gene SNC1 is an enhancer of effector-triggered immunity in Arabidopsis
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DOI：
10.1093/plphys/kiac543
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发表时间：
2023-02-12
期刊：
影响因子：
7.4
通讯作者：
Hua，Jian
中科院分区：
文献类型：
--
作者：
Wang，Zhixue;Yang，Leiyun;Hua，Jian
植物中含有许多核苷酸结合的富含亮氨酸重复序列（NLR）蛋白，这些蛋白被认为是细胞内免疫受体，但在植物-病原体相互作用过程中还没有确定的功能。NPR 1 -1的供应者，组成1（SNC 1）是Toll-白细胞介素1受体（TIR）类型的一种这样的NLR蛋白，尽管其充分表征的功能获得活性和其参与拟南芥（Arabidopsis thaliana）中的自身免疫。在这里，我们研究了SNC 1在自然植物-病原体相互作用中的作用，并从遗传学上测试了其TIR结构域的酶活性对其功能的重要性。SNC 1功能缺失突变体对含有特异性效应子的铜绿假单胞菌无毒病原菌菌株更敏感，尤其是在恒定光照条件下。在恒定光照条件下，突变体对非致病性病原菌的诱导表达和过敏反应也有所降低。此外，遗传和生物化学研究支持SNC 1的TIR酶活性是其功能获得活性所必需的。总之，我们的研究揭示了SNC 1在自然植物-病原体相互作用期间作为植物防御反应放大器的作用，并表明其使用酶活性和分子间相互作用来触发自身免疫反应。
Plants contain many nucleotide-binding leucine-rich repeat (NLR) proteins that are postulated to function as intracellular immune receptors but do not yet have an identified function during plant–pathogen interactions. SUPPRESSOR OF NPR1-1, CONSTITUTIVE 1 (SNC1) is one such NLR protein of the Toll-interleukin 1 receptor (TIR) type, despite its well-characterized gain-of-function activity and its involvement in autoimmunity in Arabidopsis (Arabidopsis thaliana). Here, we investigated the role of SNC1 in natural plant–pathogen interactions and genetically tested the importance of the enzymatic activities of its TIR domain for its function. TheSNC1loss-of-function mutants were more susceptible to avirulent bacterial pathogen strains ofPseudomonas syringaecontaining specific effectors, especially under constant light growth condition. The mutants also had reduced defense gene expression induction and hypersensitive responses upon infection by avirulent pathogens under constant light growth condition. In addition, genetic and biochemical studies supported that the TIR enzymatic activity of SNC1 is required for its gain-of-function activity. In sum, our study uncovers the role of SNC1 as an amplifier of plant defense responses during natural plant–pathogen interactions and indicates its use of enzymatic activity and intermolecular interactions for triggering autoimmune responses.