Low-level determination of six arsenic species in urine by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry (HPLC-ICP-MS).
Low-level determination of six arsenic species in urine by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry (HPLC-ICP-MS).
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采用高效液相色谱-电感耦合等离子体质谱 (HPLC-ICP-MS) 测定尿液中六种低浓度的砷。
DOI：
10.1039/c0ay00601g
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Nriagu,JeromeO
中科院分区：
文献类型：
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作者：
Rivera-Núñez,Zorimar;Linder,AaronM;Chen,Bin;Nriagu,JeromeO
许多用于分析尿液中砷代谢物形态的方法无法充分解析亚砷酸盐 (As[III]) 和砷甜菜碱 (AsB) 的色谱峰。我们提出了一种高效液相色谱-电感耦合等离子体质谱 (HPLC-ICP-MS) 方法，该方法经过优化，可以可靠地测量人尿液中的以下六种砷：As[III]、砷酸盐 (As[V])、一甲基胂酸 (MMA[III])、一甲基胂酸 (MMA[V])、二甲基胂酸 (DMA[V]) 和 AsB。该方法根据流动相的变化、准确度和精密度进行评估。定量给定样品中六种物质的能力取决于方法的低检测限：AsB 为 0.06 µg L−1，As[III] 为 0.11 µg L−1，DMA[V] 为 0.08 µg L−1，MMA[V] 为 0.12 µg L−1，As[V] 为 0.15 µg L−1。该程序用于测量密歇根州东南部 387 名长期接触砷含量略高的饮用水的人的尿液样本中的六种砷。 99.2% 的样品检测到 DMA[V]，98.2% 的 AsB，73.4% 的 MMA[V]，45.0% 的 As[III]，27.1% 的 As[V]。即使在采集后 6 小时内分析的样品中也未检测到 MMA[III]。结果引起了人们的一些疑问：对于饮用水中砷浓度低于 20 µg L−1 的人来说，MMA[III] 是否是尿液中的一种重要代谢物。
Many methods that have been used to speciate arsenic metabolites in urine are unable to adequately resolve the chromatographic peaks for arsenite (As[III]) and arsenobetaine (AsB). We present a High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry (HPLC-ICP-MS) method that has been optimized to reliably measure the following six arsenic species in human urine: As[III], arsenate (As[V]), monomethylarsonous acid (MMA[III]), monomethylarsonic acid (MMA[V]), dimethylarsinic acid (DMA[V]) and AsB. The method was evaluated with regards to changes in mobile phase, accuracy and precision. The ability to quantify the six species in a given sample depended on the low detection limits of the method—0.06 µg L−1 for AsB, 0.11 µg L−1 for As[III], 0.08 µg L−1 for DMA[V], 0.12 µg L−1 for MMA[V] and 0.15 µg L−1 for As[V]. The procedure was used to measure the six arsenic species in urine samples from 387 individuals in southeast Michigan who are chronically exposed to slightly elevated levels of arsenic in their drinking water. The DMA[V] was detected in 99.2% of samples, AsB in 98.2%, MMA[V] in 73.4%, As[III] in 45.0%, and As[V] in 27.1%. No MMA[III] was detected even in samples analyzed within 6 hours after collection. The results raise some doubt as to whether MMA[III] is a significant metabolite in urine of people exposed to arsenic concentrations below 20 µg L−1 in their drinking water.