Comparison of Macro-and Micro-porosity of a Titanium Mesh for Guided Bone Regeneration: An In Vivo Experimental Study
Comparison of Macro-and Micro-porosity of a Titanium Mesh for Guided Bone Regeneration: An In Vivo Experimental Study
复制标题
DOI：
10.21873/invivo.12678
复制
发表时间：
2022-01-01
期刊：
影响因子：
2.3
通讯作者：
Sato, Shuichi
中科院分区：
文献类型：
--
作者：
Senoo, Motoki;Hasuike, Akira;Sato, Shuichi
背景/目的：引导骨再生（Guided bone regeneration， GBR）是在种植体植入前进行垂直嵴增强的一种手术方法。钛网已被临床医生用于GBR部位的成骨空间维持。比较钛网微孔和大孔对大鼠颅骨垂直GBR模型骨再生的影响。材料与方法：9只大鼠颅骨外露，双侧置塑料瓶。18个手术部位根据钛盖和骨替代物的材料随机分为3组：微孔钛盖+脱蛋白牛骨矿物（DBBM）、大孔钛盖+DBBM、微孔钛盖+碳酸盐磷灰石。使用微型计算机断层扫描（micro-CT）评估柱内新生成的骨。此外，在12周时进行骨再生和血管生成的组织学评估。结果：显微ct定量体积分析显示，三组骨柱内骨体积逐渐增加。组织学观察证实，与大孔组相比，微孔组骨再生旺盛。在圆柱体的上部，软组织通过大孔网的孔隙侵入GBR部位。微孔组的上半部分血管明显小于大孔组。填充DBBM和碳酸盐磷灰石的骨柱在骨形成方面没有差异。结论：微血管可穿透直径50 μ m的骨柱内微孔，促进骨形成。微孔钛网可促进硬脑膜和骨膜血管生成。我们的数据显示微孔钛具有血管渗透性和骨导电性的优势，支持骨生长。
Background/Aim: Guided bone regeneration (GBR) is one of the surgical methods used for vertical ridge augmentation prior to dental implant placements. Titanium meshes have been used for osteogenic space maintenance in GBR sites by clinicians. We aimed to compare the influence of micropores and macropores in a titanium mesh on bone regeneration in a rat calvarial vertical GBR model. Materials and Methods: The calvaria of nine rats were exposed, and plastic cylinders were set bilaterally. Eighteen surgical sites were randomly allocated into three groups according to the materials of titanium lid and bone substitutes: microporous titanium lid+deproteinized bovine bone mineral (DBBM), macroporous titanium lid +DBBM, microporous titanium lid+carbonate apatite. Newly generated bone inside the cylinders was evaluated using micro-computed tomography (micro-CT). Furthermore, bone regeneration and angiogenesis were evaluated histologically at 12 weeks. Results: Quantitative volumetric analyses using micro-CT showed a gradual increase in bone volume inside the cylinders in all three groups. Histological observation confirmed vigorous bone regeneration in the microporous groups compared to that in the macroporous group. In the upper part of the cylinders, soft tissue invaded the GBR site by passing through the pores of the macroporous mesh. The blood vessels in the upper part of the cylinders were smaller in the microporous groups than in the macroporous group. There was no difference in bone formation between cylinders filled with DBBM or carbonate apatite. Conclusion: Microvasculature penetrates 50-mu m diameter micropores and accelerates bone formation inside the cylinder, which was set on rat calvaria. The microporous titanium mesh can facilitate angiogenesis from both the dura mater and periosteal in vertical ridge augmentation. Our data showed superiority of microporous titanium vascular permeability and osteoconductivity, supporting bone growth.