Interleukin-6: effects on and production by rat granulosa cells in vitro.
Interleukin-6: effects on and production by rat granulosa cells in vitro.
复制标题
DOI：
10.1210/endo.130.3.1537322
复制
发表时间：
1992-03
期刊：
影响因子：
4.8
通讯作者：
W. Gorospe;F. Hughes;B. Spangelo
中科院分区：
文献类型：
--
作者：
W. Gorospe;F. Hughes;B. Spangelo
FSH刺激的颗粒细胞与增加量的白细胞介素-6（IL-6）的治疗引起了显着的浓度依赖性抑制孕酮的生物合成。然而，在非FSH刺激的细胞中的基础孕酮产生仍然对细胞因子无反应。通过7 TD 1杂交瘤细胞生物测定法对来自未处理的颗粒细胞的条件培养基中的IL-6进行定量，揭示了可检测水平的IL-6。此外，FSH处理引起IL-6释放的显著浓度依赖性增加。与此相反，基础和FSH刺激的IL-6释放可以显着抑制干扰素γ（INF-γ）。本研究的结果表明：1）IL-6在调节孕酮产生中的作用，2）颗粒细胞是IL-6的来源，3）颗粒细胞释放IL-6是一个受调节的事件。
Treatment of FSH-stimulated granulosa cells with increasing amounts of interleukin-6 (IL-6) caused a significant concentration-dependent suppression of progesterone biosynthesis. However, basal progesterone production in non-FSH-stimulated cells remained unresponsive to the cytokine. Quantitation of IL-6 in the conditioned media from untreated granulosa cells by the 7TD1 hybridoma cell bioassay revealed detectable levels of IL-6. Further, FSH treatment caused a significant concentration-dependent increase in IL-6 release. In contrast, both basal and FSH-stimulated IL-6 release could be significantly suppressed by interferon gamma (INF-gamma). The results of the present study suggest: 1) a role for IL-6 in the regulation of progesterone production, 2) that the granulosa cell is a source of IL-6 and 3) that the release of IL-6 by the granulosa cell is a regulated event.