Stimulation of Keratinocyte Migration by Growth Factors
Stimulation of Keratinocyte Migration by Growth Factors
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DOI：
10.1111/j.1346-8138.1992.tb03751.x
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发表时间：
1992-11
期刊：
影响因子：
--
通讯作者：
R. Tsuboi;C. Sato;C. Shi;H. Ogawa
中科院分区：
文献类型：
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作者：
R. Tsuboi;C. Sato;C. Shi;H. Ogawa
角质形成细胞从伤口边缘的迁移被认为是上皮再生的关键特征之一。使用正常人角质形成细胞进行定量迁移测定。将接种在12孔板上的角质形成细胞在含有0.08mM Ca++的无血清角质形成细胞生长培养基（KGM，Curabo Co）中生长。在创伤前6小时将培养基从KGM转换为角质形成细胞基础培养基（KBM）。用剃刀刀片去除板的角化细胞的汇合单层的一半，并将剩余的角化细胞在指定生长因子存在下在KBM中孵育16小时。孵育后，将细胞固定并在100倍放大下计数。通过计数10个连续125 μm区域中的细胞数量来定量迁移。
Migration of keratinocytes from the wound edge is thought to be one of the critical features of reepithelialization. A quantitative migration assay was carried out using normal human keratinocytes. Keratinocytes, seeded on 12 well plates, were grown in serum free, keratinocyte growth medium (KGM, Curabo Co) with 0.08mM Ca++. The medium was switched from KGM to keratinocyte basal medium (KBM) 6 h prior to the wounding. Half of the plate's confluent monolayer of keratinocytes was removed with a razor blade, and the remaining keratinocytes were incubated in KBM for 16 hrs in the presence of indicated growth factors. After incubation, the cells were fixed and counted at 100 magnification. Migration was quantitated by counting the number of cells in ten successive 125‐μm zones.