Distinct roles for two G protein alpha subunits in fungal virulence, morphology, and reproduction revealed by targeted gene disruption
Distinct roles for two G protein alpha subunits in fungal virulence, morphology, and reproduction revealed by targeted gene disruption
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DOI：
10.1073/pnas.93.24.14122
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发表时间：
1996-11-26
影响因子：
11.1
通讯作者：
Nuss, DL
中科院分区：
文献类型：
--
作者：
Gao, SJ;Nuss, DL
由于低病毒感染或转基因共抑制，GTP结合蛋白G（i）α亚基CPG-1的积累减少，与栗疫病菌Cryphonectria parasitica的毒力衰减相关。通过靶向破坏编码CPG-1的基因cpg-1和编码亚基CPG-2的第二个G α基因cpg-2，进一步检查了G蛋白介导的信号转导在真菌毒力中的作用。cpg-1的破坏导致一组表型变化，其类似于与低病毒感染相关的表型变化，但比其更严重，变化包括真菌生长速率的显著降低和毒力的丧失、无性孢子形成、雌性育性和编码漆酶的基因lac-1的转录诱导。cpg-2的破坏仅导致生长速率和无性孢子形成的轻微降低，而毒力、雌性生育力或lac-1 mRNA诱导没有显著降低。这些结果提供了对先前相关证据的明确确认，所述相关证据表明许多真菌过程（包括毒力和繁殖）需要CPG-1相关的信号传导，同时证明第二G α，CPG-2，对于这些过程是不稳定的。它们还显著加强了对低病毒介导的G蛋白信号转导的破坏和真菌毒力的减弱之间的明显联系的支持。
Reduced accumulation of the GTP-binding protein G(i) alpha subunit CPG-1, due either to hypovirus infection or transgenic cosuppression, correlates with virulence attenuation of the chestnut blight fungus, Cryphonectria parasitica. The role of G protein-mediated signal transduction in fungal virulence was further examined by targeted disruption of the gene cpg-1, encoding CPG-1, and a second G alpha gene, cpg-2, encoding the subunit CPG-2, Disruption of cpg-1 resulted in a set of phenotypic changes similar to, but more severe than, those associated with hypovirus infection, Changes included a marked reduction in fungal growth rate and loss of virulence, asexual sporulation, female fertility, and transcriptional induction of the gene lac-1, encoding the enzyme laccase, In contrast, cpg-2 disruption resulted in only slight reductions in growth rate and asexual sporulation and no significant reduction in virulence, female fertility, or lac-1 mRNA inducibility. These results provide definitive confirmation of previous correlative evidence that suggested a requirement of CPG-l-linked signaling for a number of fungal processes, including virulence and reproduction, while demonstrating that a second G alpha, CPG-2, is dispensable for these processes, They also significantly strengthen support for the apparent linkage between hypovirus-mediated disruption of G protein signal transduction and attenuation of fungal virulence.