31-P nuclear magnetic resonance studies on serum low and high density lipoproteins: effect of paramagnetic ion.
31-P nuclear magnetic resonance studies on serum low and high density lipoproteins: effect of paramagnetic ion.
复制标题
31-P核磁共振对血清低密度脂蛋白和高密度脂蛋白的研究：顺磁离子的影响。
作者：
T. Henderson;A. W. Kruski;L. G. Davis;T. Glonek;A. Scanu
本文报道了一种顺磁性猝灭剂Mn ~（2+）/EDTA（1：2.2）对人血清低密度脂蛋白和高密度脂蛋白的~（31）P核磁共振信号的猝灭作用。研究的系统包括天然低密度和高密度血清脂蛋白（LDL，HDL 2和HDL 3），卵磷脂囊泡连同适当的磷酸二酯模型系统，磷酸二乙酯在水性缓冲液中，和磷脂酰胆碱和鞘磷脂都在无水甲醇。这些研究结果表明，CA。与HDL 2和HDL 3观察到的80- 85%的数字相比，LDL的50%的磷脂-磷信号在滴定时被淬灭。在所有情况下，通过透析去除顺磁性离子，光谱效应是完全可逆的。滴定研究的结果表明HDL 2和HDL 3之间的行为相似但不完全相同。结果与低密度脂蛋白和高密度脂蛋白颗粒的低角X射线衍射模型结构一致。
A paramagnetic quenching reagent, Mn-2+/EDTA (1:2.2), was developed for the purpose of investigating the phospholipid phosphate groupings of human serum low and high density lipoproteins through the quenching effect of the reagent on the 31-P nuclear magnetic resonance signals from these complexes. Systems investigated included native low and high density serum liproteins (LDL, HDL2, and HDL3), egg phosphatidylcholine vesicles together with appropriate phosphodiester model systems, diethyl phosphate in aqueous buffer, and phosphatidylcholine and sphingomyelin both in anhydrous methanol. The results of these studies indicated that ca. 50 percent of the phospholipid-phosphorus signal of LDL is quenched upon titration as compared to an 80-85 percent figure observed for HDL2 and HDL3. In all cases the spectral effects were totally reversible upon removalof the paramagnetic ion by dialysis. The results of the titration studies indicated a similar but not an identical behavior between HDL2 and HDL3. The results are consistent with model structures of HDL and LDL particles derived from low angle X-ray diffraction.