CLONAL HETEROGENEITY IN THE FUNCTIONAL REQUIREMENT FOR LYT-2/3 MOLECULES ON CYTOLYTIC LYMPHOCYTES-T (CTL) - POSSIBLE IMPLICATIONS FOR THE AFFINITY OF CTL ANTIGEN RECEPTORS
CLONAL HETEROGENEITY IN THE FUNCTIONAL REQUIREMENT FOR LYT-2/3 MOLECULES ON CYTOLYTIC LYMPHOCYTES-T (CTL) - POSSIBLE IMPLICATIONS FOR THE AFFINITY OF CTL ANTIGEN RECEPTORS
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DOI：
10.1111/j.1600-065x.1982.tb01061.x
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发表时间：
1982-01-01
影响因子：
8.7
通讯作者：
CEROTTINI, JC
中科院分区：
文献类型：
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作者：
MACDONALD, HR;GLASEBROOK, AL;CEROTTINI, JC
尽管在过去十年中进行了广泛的研究，但细胞溶解性T淋巴细胞（CTL）裂解靶细胞的机制仍然不清楚（最近的评论，参见Perlmann & Cerottini 1979， Berke 1980）。由于已知裂解需要CTL和靶细胞之间的密切接触，因此解决这一问题的一个有希望的方法是寻找CTL上可能在抗原识别和/或细胞裂解活性表达中发挥作用的表面分子。随着最近单克隆抗体的可用性，凭借相应的单克隆抗体在缺乏补体的情况下阻断CTL活性的能力，鉴定这些潜在的有趣的表面分子已经成为可能。利用这种方法，最近在CTL群体上发现了两种可能的“功能性”分子复合物：Lyt-2/3 （Nakayama et al. 1979, Shinohara et al. 1979）和LFA-1 （Davignon et al. 1981）。对这些抗体阻断实验的解释至少有两个限制。首先，对凝集素或同种异体抗原激活的多克隆CTL群体进行阻断；因此，不可能
Despite extensive investigation over the past decade, the mechanism of target cell lysis by cytolytic T lymphocytes (CTL) remains obscure (for recent reviews, see Perlmann & Cerottini 1979, Berke 1980). Since lysis is known to require close contact between CTL and target cells, one promising approach to this problem is the search for surface molecules on CTL which may play a role in antigen recognition and/or the expression of cytolytic activity. With the recent availability of monoclonal antibodies, it has become possible to identify such potentially interesting surface molecules by virtue of the ability of a corresponding monoclonal antibody to block CTL activity in the absence of complement. Using such an approach, two possible" functional" molecular complexes on CTL populations have been recently identified: Lyt-2/3 (Nakayama et al. 1979, Shinohara et al. 1979) and LFA-1 (Davignon et al. 1981). At least two limitations to the interpretation of these antibody blocking experiments can be invoked. Firstly, blocking was carried out on polyclonal CTL populations activated by lectins or alloantigens; thus it is not possible to