LOCALIZED CONVERSION AT THE CROSSOVER SEQUENCES IN THE SITE-SPECIFIC DNA INVERSION SYSTEM OF BACTERIOPHAGE-P1
LOCALIZED CONVERSION AT THE CROSSOVER SEQUENCES IN THE SITE-SPECIFIC DNA INVERSION SYSTEM OF BACTERIOPHAGE-P1
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DOI：
10.1016/0092-8674(86)90539-8
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发表时间：
1986-04-11
期刊：
影响因子：
64.5
通讯作者：
HIESTANDNAUER, R
中科院分区：
文献类型：
--
作者：
IIDA, S;HIESTANDNAUER, R
位点特异性C反转的交叉位点由不完美的12bp反向重复组成，二核苷酸TT位于对称中心。P1 Cin重组酶的噬菌体不仅作用于这些cix位点，而且作用于cix相关序列（称为准cix位点，cixQ）的效率较低。当cixQ含有中心二核苷酸TT时，体内正常位点和准cix位点的2bp序列TT发生交叉。如果cixQ只携带一个T残基，反转相关的局部转换可能发生在2bp序列内的不匹配位置。结果表明，Cin在体内产生2bp的交错切割，在这2bp的交叉序列上发生了互惠链交换。
The crossover sites for site-specific C inversion consist of imperfect 12 bp inverted repeats with the dinucleotide TT at the center of symmetry. The phage of P1 Cin recombinase acts not only at these cix sites but also less efficiently at cix-related sequences called quasi-cix sites, cixQ. When cixQ contains a central dinucleotide TT, crossover occurs in vivo a the 2 bp sequence TT in the normal and the quasi-cix sites. If cixQ carries only one T residue, inversion-associated localized conversion can occur at the mismatched position within the 2 bp sequence. The results indicate that Cin generates 2 bp staggered cuts in vivo and that reciprocal strand exchanges occur at these 2 bp crossover sequences.