Induction of an LH surge and ovulation by buserelin (as Receptal) allows breeding of weaned sows with a single fixed-time insemination
Induction of an LH surge and ovulation by buserelin (as Receptal) allows breeding of weaned sows with a single fixed-time insemination
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DOI：
10.1016/j.theriogenology.2013.05.002
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发表时间：
2013-09-01
期刊：
影响因子：
2.8
通讯作者：
Soede, N. M.
中科院分区：
文献类型：
--
作者：
Driancourt, M. A.;Cox, P.;Soede, N. M.
本研究的目的是证明布舍瑞林（一种GnRH类似物）诱导排卵后单次固定时间授精的母猪成功繁殖。在第一步中，使用布舍瑞林诱导与对照母猪（N = 15）相似幅度的LH峰和诱导排卵的能力，确定断奶后77小时在断奶母猪（N = 15、11和12）中注射布舍瑞林（6、10或16 μ g）的最佳剂量。在29/38头给药母猪中。(76%），在注射后32和44小时之间诱导排卵并使其同步化，并且在该时间窗期间排卵的雌性的比例在组间相似，分别为73%、73%和83%（分别为6、10或16 μ g）。有趣的是，尽管100%经产母猪的排卵在给药后32至44小时的时间窗内诱导和同步化，但在较低比例的经产母猪中成功诱导（6、10或16 μ g后分别为50%、50%和67%），剂量效应不显著。无论注射的布舍瑞林剂量如何，对照组和给药组母猪的LH峰幅度相似。排卵后第5天的排卵率和良好胚胎数量在组间均无差异。有趣的是，屠宰时卵泡囊肿的频率受到给药的显著影响（P < 0.05），在10或6 μ g布舍瑞林给药的母猪中分别为最小和最大。在第二步中，将来自西班牙、德国和法国商业牛群的419头母猪随机分配至对照组或给药组。一旦检测到发情，对照母猪授精两次，间隔12 +/- 4小时。给药母猪在断奶后86 +/- 3小时接受10 μ g布舍瑞林，并在30 - 33小时后授精一次。处理母猪的分娩率（87%，166/192）与对照母猪的分娩率（84.5%，169/200）相似。处理组和对照组母猪的窝仔数也相似（13.6 +/- 3.8 vs. 13.7 +/- 3.2）。在经产母猪中，泌乳持续时间和泌乳期间脂肪损失的幅度均不显著影响给药效果。可以得出结论，断奶后86小时布舍瑞林（10 μ g）给药可使断奶经产母猪紧密同步排卵。这允许在注射布舍瑞林后在固定时间繁殖一次，同时将繁殖性能保持在与发情期繁殖两次的母猪相似的水平。(C)2013 Elsevier Inc. All rights reserved.
The aim of this study was to demonstrate successful breeding of sows with a single fixed-time insemination following ovulation induction by buserelin, a GnRH analogue. In a first step, the optimal dose of buserelin (6,10, or 16 mu g) injected at 77 hours after weaning was determined in weaned sows (N = 15, 11, and 12, respectively) using its ability to induce an LH surge of similar magnitude as in control sows (N = 15) and induce ovulation. In 29/38 treated sows. (76%), ovulation was induced and synchronized between 32 and 44 hours after injection, and the proportion of females ovulating during this time window was similar between groups at 73%, 73%, and 83% (6, 10, or 16 mu g, respectively). Interestingly, whereas ovulation of 100% multiparous sows was induced and synchronized in the 32 to 44 hours posttreatment time window, successful induction was achieved in a lower proportion of primiparous sows (50%, 50%, and 67% following 6,10, or 16 mu g, respectively), the dose effect being nonsignificant. The magnitude of the LH surge was similar between control and treated sows, irrespective of the buserelin dose injected. Neither ovulation rate nor the number of good embryos on Day 5 postovulation differed between groups. Interestingly, the frequency of follicular cysts at slaughter was significantly affected by treatment (P < 0.05), being minimal and maximal in sows treated with 10 or 6 mu g buserelin, respectively. In a second step, 419 sows from commercial herds in Spain, Germany, and France were randomly allocated to a control or treated group. The control sows were inseminated twice 12 +/- 4 hours apart once estrus was detected. Treated sows received 10 mu g buserelin at 86 +/- 3 hours after weaning and were inseminated once 30 to 33 hours later. Farrowing rate of treated sows (87%, 166/192) was similar to that of control sows (84.5%, 169/200). Litter size was also similar between treated and control sows (13.6 +/- 3.8 vs. 13.7 +/- 3.2). In multiparous sows, neither duration of lactation nor magnitude of the fat loss during lactation significantly affected treatment effects. It is concluded that ovulation of weaned multiparous sows can be tightly synchronized by buserelin (10 mu g) administration at 86 hours postweaning. This allows breeding once at a fixed time following buserelin injection while maintaining reproductive performance at a level similar to that of sows bred twice during estrus. (C) 2013 Elsevier Inc. All rights reserved.