Antimicrobial Chemicals Are Associated with Elevated Antibiotic Resistance Genes in the Indoor Dust Microbiome.
Antimicrobial Chemicals Are Associated with Elevated Antibiotic Resistance Genes in the Indoor Dust Microbiome.
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DOI：
10.1021/acs.est.6b00262
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发表时间：
2016-09-20
影响因子：
11.4
通讯作者：
Green, Jessica L.
中科院分区：
文献类型：
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作者：
Hartmann, Erica M.;Hickey, Roxana;Hsu, Tiffany;Roman, Clarisse M. Betancourt;Chen, Jing;Schwager, Randall;Kline, Jeff;Brown, G. Z.;Halden, Rolf U.;Huttenhower, Curtis;Green, Jessica L.
抗生素耐药性越来越普遍，主要是由于人类的影响。在这里，我们探讨了抗生素耐药基因和抗菌化学品三氯生，三氯卡班，甲基，乙基，丙基和丁基对羟基苯甲酸酯在灰尘微生物组之间的关系。混合使用的运动和教育设施的灰尘样本进行微生物和化学分析，使用16 S rRNA扩增子测序，鸟枪宏基因组测序和液相色谱串联质谱法的组合。通过使用短的、更好的代表性提取物数据集（ShortBRED）从每个样品的宏基因组鉴定在综合抗生素抗性数据库（CARD）中注释的抗生素抗性基因来表征粉尘抗性组。三个最高丰度的抗生素抗性基因是泰特（W）、blaSRT-1和blaSRT（B）。然后将完整的防尘层与测得的抗菌化学品浓度进行比较，三氯生的浓度范围为0.5至1970 ng/g灰尘。我们观察到抗微生物化学品的浓度与抗生素耐药基因的相对丰度之间存在六种显著的正相关性，其中包括普遍存在的抗微生物三氯生与23 S rRNA甲基转移酶（X）之间的正相关性，这是一种与多种抗生素耐药性有关的23 S rRNA甲基转移酶。这项研究是第一个寻找抗生素耐药性基因和灰尘中抗菌化学物质之间的关联的研究。
Antibiotic resistance is increasingly widespread, largely due to human influence. Here, we explore the relationship between antibiotic resistance genes and the antimicrobial chemicals triclosan, triclocarban, and methyl-, ethyl-, propyl-, and butylparaben in the dust microbiome. Dust samples from a mixed-use athletic and educational facility were subjected to microbial and chemical analyses using a combination of 16S rRNA amplicon sequencing, shotgun metagenome sequencing, and liquid chromatography tandem mass spectrometry. The dust resistome was characterized by identifying antibiotic resistance genes annotated in the Comprehensive Antibiotic Resistance Database (CARD) from the metagenomes of each sample using the Short, Better Representative Extract Data set (ShortBRED). The three most highly abundant antibiotic resistance genes were tet(W), blaSRT-1, and erm(B). The complete dust resistome was then compared against the measured concentrations of antimicrobial chemicals, which for triclosan ranged from 0.5 to 1970 ng/g dust. We observed six significant positive associations between the concentration of an antimicrobial chemical and the relative abundance of an antibiotic resistance gene, including one between the ubiquitous antimicrobial triclosan and erm(X), a 23S rRNA methyltransferase implicated in resistance to several antibiotics. This study is the first to look for an association between antibiotic resistance genes and antimicrobial chemicals in dust.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
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作者：
通讯作者：
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影响因子：
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作者：
Fan, Xinghua;Kubwabo, Cariton;Jones-Otazo, Heather
通讯作者：
Jones-Otazo, Heather
影响因子：
4.3
作者：
Kaminski J;Gibson MK;Franzosa EA;Segata N;Dantas G;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
5.8
作者：
Edgar, Robert C.;Flyvbjerg, Henrik
通讯作者：
Flyvbjerg, Henrik
影响因子：
46.9
作者：
通讯作者：
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