MICROPHOTOMETRIC DETERMINATION OF HEMATOCRIT IN SMALL VESSELS
MICROPHOTOMETRIC DETERMINATION OF HEMATOCRIT IN SMALL VESSELS
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DOI：
10.1152/ajpheart.1983.245.1.h167
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发表时间：
1983-01-01
影响因子：
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通讯作者：
GAEHTGENS, P
中科院分区：
文献类型：
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作者：
PRIES, AR;KANZOW, G;GAEHTGENS, P
本文介绍了一种测定透照组织微血管中红细胞压积的显微光度法的评价和校准。该方法基于微血管的光密度（OD）与其血细胞比容（Hct）的关系。显微测光系统的以下要求显得至关重要：窄带单色光源与有效的假光抑制，物镜的高数值孔径和聚光镜的低数值孔径。使用视频系统来测量入射光（I 0）和透射光（I）的强度。为了将I 0和I转换为OD值，评估了校正程序以消除眩光、阴影和褪色的影响。用灌注有红细胞悬浮液的内径（ID）在13和68 μ m之间的玻璃管进行校准程序。将函数拟合到数据，将OD与ID和Hct相关联。该函数的原始数据的SD为.+-。0.02分数红细胞压积U。所提出的方法允许在微血管中的红细胞压积的连续测定以及在微血管网络内的红细胞压积分布的离线评价。
Evaluation and calibration of a microphotometric method for hematocrit determination in microvessels of transilluminated tissues is described. This method is based on the relation of the optical density (OD) of a microvessel to its hematocrit (Hct). The following requirements of the microphotometric system appear essential: narrow-band monochromatic light source with efficient false light suppression, high numerical aperture of the objective and low numerical aperture of the condensor. A video system was used to measure the intensities of incident (I0) and transmitted (I) light. For converting of I0 and I into OD values, correcting procedures were evaluated to eliminate the influence of glare, shading and fading. The calibration procedure was performed with glass tubes of inner diameter (ID) between 13 and 68 .mu.m perfused with red cell suspensions. A function was fitted to the data, correlating OD to ID and Hct. The SD of the original data from this function is .+-. 0.02 U of fractional hematocrit. The presented method allows the continuous determination of the hematocrit in a microvessel as well as the off-line evaluation of the hematocrit distribution within a microvessel network.