Next-generation sequencing of 16S rRNA for identification of invasive bacterial pathogens in a formalin-fixed paraffin-embedded placental specimen: a case report of perinatal fulminant Streptococcus pyogenes infection
Next-generation sequencing of 16S rRNA for identification of invasive bacterial pathogens in a formalin-fixed paraffin-embedded placental specimen: a case report of perinatal fulminant Streptococcus pyogenes infection
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DOI：
10.1007/s00795-021-00298-2
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发表时间：
2021-07-21
影响因子：
1.8
通讯作者：
Saito, Tsuyoshi
中科院分区：
文献类型：
--
作者：
Mariya, Tasuku;Sato, Toyotaka;Saito, Tsuyoshi
宫内感染是孕产妇死亡的最重要原因之一。在围产期急诊中，我们常常错过获得培养标本的机会。在这项研究中，我们试图检验是否可以从福尔马林固定石蜡包埋（FFPE）胎盘标本中检测到引起感染的细菌。我们检查胎盘从母体侵入性感染，导致感染性流产在妊娠18周。病例确诊为急性发热、腹痛，经3周强化抗菌药物治疗治愈。从患者阴道液和血液培养中分离出4株化脓性链球菌。所有菌株类型均为emm1/ST28。我们从FFPE胎盘标本中扩增了16S rRNA的V1-V2区域，并使用下一代测序仪（NGS）进行测序。然后对测序数据进行分类分析。我们成功地从FFPE胎盘中检测到致病菌：69.1%的主要鉴定细菌为化脓性链球菌，并检测到其他小群体的细菌。我们的研究结果揭示了NGS对FFPE胎盘16S rRNA分析的实用性。这种方法可以回顾性地揭示以前未知来源的围产期侵袭性感染。
Intrauterine infection is one of the most important causes of maternal death. In perinatal emergency, we often miss an opportunity to obtain culture specimens. In this study, we tried to examine whether we investigated whether bacteria causing infection can be detected from a formalin-fixed paraffin-embedded (FFPE) placental specimen. We examined the placenta from a maternal invasive infection that resulted in infectious abortion at 18 weeks of gestation. The case was diagnosed by acute fever and abdominal pain, and the patient was cured after 3 weeks of intensive antimicrobial treatment. Four Streptococcus pyogenes strains were isolated from vaginal fluid and blood cultures of the patient. All of the strain types were emm1/ST28. We amplified the V1-V2 region of 16S rRNA from an FFPE placental specimen and sequencing was performed using a next-generation sequencer (NGS). Taxonomic analysis was then performed for sequenced data. We succeeded in detecting causative pathogens from the FFPE placenta: 69.1% of the predominantly identified bacteria were S. pyogenes and other small populations of bacteria were detected. Our results revealed the utility of NGS for 16S rRNA analysis of an FFPE placenta. This method may reveal previous perinatal invasive infections of unknown origin retrospectively.