TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse kidney sections using silver-deposited plate
TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse kidney sections using silver-deposited plate
复制标题
使用银沉积板对小鼠肾脏切片中的卤化物/硫氰酸根阴离子和硫化氢进行 TOF-SIMS 成像
DOI：
10.1007/s00216-011-5647-2
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发表时间：
2012
影响因子：
4.3
通讯作者：
Ishii I.
中科院分区：
文献类型：
--
作者：
Akahoshi N.;Ishizaki I.;Naya M.;Maekawa T.;Yamozoe S.;Horiuchi T.;Kajimura M.;Ohashi Y.;Suematsu M.;Ishii I.
活性小分子代谢物的体内成像具有高空间分辨率和特异性，可以为了解各种疾病的病理生理提供线索。在此，我们将飞行时间二次离子质谱（TOF-SIMS）应用于新开发的镀银板上，并将其印在小鼠组织上，成功地可视化了卤化物（Cl−，Br−和I−）和假卤化物硫氰酸盐（SCN−）阴离子，这是中性粒细胞/嗜酸性过氧化物酶产生次卤酸（HOX/OX−混合物；X:（伪）卤化物）的一类底物，以及硫化氢（H2S）。将40微米冰冻的小鼠肾切片放在盖玻片上，贴于37°C预热的镀银板上，在- 10°C下孵育1小时。溅射清洗去除表面污染物后，用TOF-SIMS分析板的分布，以确定组织源性H2S的产物Br−、AgBr2−、I−、ag2−、SCN−以及S2−和AgS−。Br−、AgBr2−、I−和SCN−阴离子主要分布在外髓质皮层和外髓质外条等核心区域（I−除外），而非外髓质皮层和外条等外区。ag2−阴离子分布在整个肾脏，尽管其水平相对较低。相反，S2 -和AgS -阴离子主要存在于外区。据我们所知，这是第一次成像研究揭示（伪）卤化物和H2S在动物组织切片中的分布。
In vivo imaging of reactive small molecule metabolites with high spatial resolution and specificity could give clues to understanding pathophysiology of various diseases. We herein applied time of flight-secondary ion mass spectrometry (TOF-SIMS) to newly developed silver-deposited plates that were stamped on mouse tissues, and succeeded in visualization of halide (Cl−, Br−, and I−) and pseudohalide thiocyanate (SCN−) anions, a class of substrates for neutrophils/eosinophil peroxidases to produce hypohalous acids (HOX/OX−mixture; X: (pseudo)halides), as well as hydrogen sulfide (H2S). Forty-micrometer frozen mouse kidney sections on cover glasses were attached to 37 °C preheated silver-deposited plates and incubated at −10 °C for 1 h. After sputter cleaning to remove surface contaminants, the plates were analyzed by TOF-SIMS to identify distribution of Br−, AgBr2−, I−, AgI2−, SCN−, as well as S2−and AgS−as products of tissue-derived H2S. Br−, AgBr2−, I−, and SCN−anions were mainly distributed in core regions including the inner medulla and inner stripe of the outer medulla (except for I−), rather than outer regions such as the cortex and outer stripe of the outer medulla. AgI2−anion was spread over the whole kidney, although its levels were relatively low. In contrast, S2−and AgS−anions were mainly present in the outer regions. To our knowledge, this is the first imaging study to reveal the distribution of (pseudo)halides and H2S in animal tissue sections.