Biodegradation of Aldrin and Dieldrin by the White-Rot Fungus Pleurotus ostreatus
Biodegradation of Aldrin and Dieldrin by the White-Rot Fungus Pleurotus ostreatus
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DOI：
10.1007/s00284-016-1184-8
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发表时间：
2017-01
影响因子：
2.6
通讯作者：
Adi Setyo Purnomo;R. Nawfa;F. Martak;Kuniyoshi Shimizu;Ichiro Kamei
中科院分区：
文献类型：
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作者：
Adi Setyo Purnomo;R. Nawfa;F. Martak;Kuniyoshi Shimizu;Ichiro Kamei
奥尔德林及其代谢物狄氏剂是一种持久性有机污染物，污染着世界上许多地区的土壤。考虑到与这些污染物相关的潜在危害，需要一种有效的降解方法。在本研究中，我们研究了平菇在纯液体培养中转化艾氏和狄氏的能力。该真菌在14天的培养期内完全消除了马铃薯葡萄糖肉汤（PDB）培养基中的aldrin。主要代谢物为狄氏剂，代谢物为9-羟基艾氏剂和9-羟基狄氏剂。提出的aldrin生物转化途径是通过环氧化进行初始代谢，然后是羟基化。这种真菌还能够降解狄氏剂，一种顽固的艾氏剂代谢物。大约3,9和18%的狄氏剂被p消除。分别在低氮、高氮和PDB培养基中孵育14天。在PDB培养物中检测到9-二羟基狄氏酮作为代谢物，表明羟基化反应发生在环氧环上。这些结果表明p。ostreatus在aldrin和dieldrin的转化中都有潜在的应用。
Aldrin and its metabolite dieldrin are persistent organic pollutants that contaminate soil in many parts of the world. Given the potential hazards associated with these pollutants, an efficient degradation method is required. In this study, we investigated the ability ofPleurotus ostreatusto transform aldrin as well as dieldrin in pure liquid cultures. This fungus completely eliminated aldrin in potato dextrose broth (PDB) medium during a 14-day incubation period. Dieldrin was detected as the main metabolite, and 9-hydroxylaldrin and 9-hydroxyldieldrin were less abundant metabolites. The proposed route of aldrin biotransformation is initial metabolism by epoxidation, followed by hydroxylation. The fungus was also capable of degrading dieldrin, a recalcitrant metabolite of aldrin. Approximately 3, 9, and 18% of dieldrin were eliminated byP. ostreatusin low-nitrogen, high-nitrogen, and PDB media, respectively, during a 14-day incubation period. 9-Dihydroxydieldrin was detected as a metabolite in the PDB culture, suggesting that the hydroxylation reaction occurred in the epoxide ring. These results indicate thatP. ostreatushas potential applications in the transformation of aldrin as well as dieldrin.