TERMINAL DIFFERENTIATION OF CULTURED HUMAN EPIDERMAL-CELLS
TERMINAL DIFFERENTIATION OF CULTURED HUMAN EPIDERMAL-CELLS
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DOI：
10.1016/0092-8674(77)90058-7
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
GREEN, H
在细胞培养中研究了在致死剂量照射的3T3细胞存在下表皮角质形成细胞的终末分化的三个方面：洗涤剂不溶性细胞质丝的发展、皮质细胞包膜的形成和细胞核的破坏。早期对完整皮肤的研究表明，表皮和真皮之间的化学梯度可能是表皮细胞分化的原因。在表面培养中，基底细胞增殖，非基底细胞经历终末分化，即使所有细胞都浸泡在相同的培养基中，并且终末分化细胞比基底细胞更容易进入培养基。几乎所有均匀暴露于培养基的单个悬浮细胞也开始分化。因此，分化程序在细胞环境中是独立的。细胞与细胞的接触对于洗涤剂不溶解性的形成、角质化包膜的形成或核消化过程并不需要，尽管它们对于扁平鳞片的形成至关重要。与强烈依赖于成纤维细胞产物的增殖不同，终末分化在没有成纤维细胞支持的情况下进行。
Three aspects of terminal differentiation of the epidermal keratinocyte in the presence of lethally irradiated 3T3 cells were studied in cell culture: the development of detergent-insoluble cytoplasmic filaments, the formation of a cornified cell envelope and the destruction of the cell nucleus. Earlier studies on intact skin suggested that chemical gradients between epidermis and dermis might be responsible for the differentiation of the epidermal cells. In surface culture, basal cells multiply and nonbasal cells undergo terminal differentiation, even though all the cells are bathed in the same medium and the terminally differentiating cells have, if anything, better access to the medium than do the basal cells. Differentiation also begins in virtually all singly suspended cells uniformly exposed to the medium. The program of differentiation is therefore independent in the cellular environment. Cell-cell contacts are not required for the development of detergent insolubility, the formation of the cornified envelope or the process of nuclear digestion, although they are essential for the formation of flattened squames. Unlike proliferation, which is strongly dependent upon fibroblast products, terminal differentiation proceeds in the absence of fibroblast support.