Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
复制标题
马里帕鲁迪斯甲烷球菌：具有多种功能 MCM 蛋白的古细菌？
DOI：
10.1042/bst0370001
复制
发表时间：
2009
影响因子：
3.9
通讯作者：
J. Chong
中科院分区：
文献类型：
--
作者：
Alison D. Walters;J. Chong
有大量的蛋白质参与真核DNA复制的控制，它们共同作用以确保DNA在每个细胞周期仅复制一次。参与DNA复制的起始和延伸阶段的关键蛋白包括MCM（小染色体维持）蛋白，MCM2-MCM7，一个被认为充当复制解旋酶的六个相关蛋白的家族。基因组测序显示，古生菌拥有一套简化的真核复制同源物。真核生物中DNA复制机制的复杂性导致了许多古细菌物种被改编为研究DNA复制过程的模式生物。大多数古细菌测序日期拥有一个单一的MCM同源物，形成一个六聚体复合物。从几个古细菌的重组MCM已被用于蛋白质的生化特性，揭示MCM复合物具有ATP酶，DNA结合和解旋活动。不寻常的是，产甲烷古菌Methanococcus maripaludis的基因组包含四个MCM同源物，所有这些同源物都包含功能所需的保守基序。广泛的遗传工具的可用性，用于操纵M。maripaludis和这种生物在实验室中相对容易生长，使其成为研究多个MCMs在DNA复制中作用的良好潜在模型。
There are a large number of proteins involved in the control of eukaryotic DNA replication, which act together to ensure DNA is replicated only once every cell cycle. Key proteins involved in the initiation and elongation phases of DNA replication include the MCM (minchromosome maintenance) proteins, MCM2-MCM7, a family of six related proteins believed to act as the replicative helicase. Genome sequencing has revealed that the archaea possess a simplified set of eukaryotic replication homologues. The complexity of the DNA replication machinery in eukaryotes has led to a number of archaeal species being adapted as model organisms for the study of the DNA replication process. Most archaea sequenced to date possess a single MCM homologue that forms a hexameric complex. Recombinant MCMs from several archaea have been used in the biochemical characterization of the protein, revealing that the MCM complex has ATPase, DNA-binding and -unwinding activities. Unusually, the genome of the methanogenic archaeon Methanococcus maripaludis contains four MCM homologues, all of which contain the conserved motifs required for function. The availability of a wide range of genetic tools for the manipulation of M. maripaludis and the relative ease of growth of this organism in the laboratory makes it a good potential model for studying the role of multiple MCMs in DNA replication.
DOI：
10.1073/pnas.030539597
发表时间：
2000-02-15
影响因子：
11.1
作者：
Chong, JPJ;Hayashi, MK;Stillman, B
通讯作者：
Stillman, B
DOI：
10.1073/pnas.0507479102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Snyder, M;He, W;Zhang, JJ
通讯作者：
Zhang, JJ