Expression of genes in gastrointestinal and lymphatic tissues during parasite infection in sheep genetically resistant or susceptible to Trichostrongylus colubriformis and Haemonchus contortus
Expression of genes in gastrointestinal and lymphatic tissues during parasite infection in sheep genetically resistant or susceptible to Trichostrongylus colubriformis and Haemonchus contortus
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DOI：
10.1016/j.ijpara.2009.09.007
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发表时间：
2010-03-15
影响因子：
4
通讯作者：
Windon, Ross
中科院分区：
文献类型：
--
作者：
Andronicos, Nicholas;Hunt, Peter;Windon, Ross
对急性胃肠道线虫（GIN）感染的抵抗力取决于宿主识别寄生虫并产生保护性Th 2应答的能力。据推测，遗传易感GIN的羔羊将差异上调Th 1型基因，因此与遗传抗性羔羊相比，仍然容易受到慢性寄生虫感染，这将差异上调Th 2型基因，清除寄生虫感染。两个选择羊群，其中线的美利奴羊产生的羔羊遗传抗性或易感GIN，急性挑战一次或三次，无论是捻转血矛线虫或Trichostrongylus colubriformis。粪便虫卵计数（FEC），血浆和组织抗寄生虫（H。contortus或T. colubriformis）抗体同种型反应显示抗性动物用T. colubriformis建立了保护性Th 2应答（可忽略的FEC、IgG 1和IgE），而易感动物需要多次攻击以建立显著的IgG 1应答，尽管FEC保持高水平。蛇形毛圆线虫比H.扭曲我们通过微阵列和定量PCR分析询问从每个寄生虫感染部位和相关淋巴结的组织中提取的RNA，以将宿主基因表达与FEC和抗体应答相关联。IFN-γ诱导基因cxcl 10在毛圆线虫选择群绵羊的易感系中在用寄生虫H. contortus和T.游蛇状的然而，一个统一的模式的基因没有上调的抗性动物从两个选择羊群在两个寄生虫感染，这表明这些寄生虫的宿主抗性的模式是不同的，虽然在主机的易感性有一些相似之处是显而易见的。皇冠版权所有（C）2009年由爱思唯尔有限公司代表澳大利亚寄生虫学会出版。All rights reserved.
Resistance to an acute gastrointestinal nematode (GIN) infection is dependent on the ability of the host to recognise the parasite and mount a protective Th2 response. It is hypothesised that lambs which are genetically susceptible to GIN will differentially up-regulate Th1-type genes and therefore remain susceptible to chronic parasitism compared with genetically resistant lambs which will differentially up-regulate Th2-type genes and clear the parasite infection. Two selection flocks, in which lines of Merino sheep produced lambs genetically resistant or susceptible to GIN, were acutely challenged once or thrice with either Haemonchus contortus or Trichostrongylus colubriformis. Faecal-egg counts (FECs), and plasma and tissue anti-parasite (H. contortus or T. colubriformis) antibody isotype responses showed that resistant animals challenged three times with T. colubriformis established a protective Th2 response (negligible FEC, IgG1 and IgE) whereas susceptible animals required multiple challenges to establish a significant IgG1 response despite FECs remaining high. Trichostrongylus colubriformis elicited a more pronounced host response than H. contort us. RNA extracted from tissues at the site of each parasite infection and associated lymph nodes were interrogated by microarray and quantitative PCR analyses to correlate host gene expression to FECs and antibody responses. The IFN-gamma inducible gene cxcl10 was up-regulated in the susceptible line of the Trichostrongylus selection flock sheep after a tertiary challenge with the parasites H. contortus and T. colubriformis. However, a uniform pattern of genes was not up-regulated in resistant animals from both selection flocks during both parasite infections, suggesting that the mode of host resistance to these parasites is different, although some similarities in host susceptibility were apparent. Crown Copyright (C) 2009 Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc. All rights reserved.