IDENTIFICATION OF MYCOBACTERIA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
IDENTIFICATION OF MYCOBACTERIA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1128/jcm.29.11.2468-2472.1991
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发表时间：
1991-11-01
影响因子：
9.4
通讯作者：
KILBURN, JO
中科院分区：
文献类型：
--
作者：
BUTLER, WR;JOST, KC;KILBURN, JO
从皂化的分枝杆菌细胞中提取的分枝杆菌酸通过高效液相色谱法（HPLC）检测为对溴苯甲酰甲基酯。 通过检查培养物收集的菌株和其他充分表征的分离株，开发了分枝杆菌种属的标准HPLC图谱。 使用峰的相对保留时间和峰高比较来开发鉴别方案，该方案对于检查的物质的准确度为98%。 本文介绍了一种快速、经济高效的HPLC方法，为分枝杆菌的鉴定提供了一种替代方法。
Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.