Fluoro-Jade: A novel fluorochrome for the sensitive and reliable histochemical localization of neuronal degeneration
Fluoro-Jade: A novel fluorochrome for the sensitive and reliable histochemical localization of neuronal degeneration
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DOI：
10.1016/s0006-8993(96)01387-x
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发表时间：
1997-03-14
期刊：
影响因子：
2.9
通讯作者：
Slikker, W
中科院分区：
文献类型：
--
作者：
Schmued, LC;Albertson, C;Slikker, W
Fluoro-Jade是一种阴离子荧光染料，能够选择性地染色脑切片中的退化神经元。荧光玉的组织化学应用为变性神经元及其过程的染色提供了一种简单、灵敏、可靠的方法。该技术将检测由于暴露于各种神经毒性损伤而导致的神经元变性。Fluoro-Jade可以与其他荧光方法结合使用，包括免疫荧光、荧光轴突束示踪和荧光尼氏反染。与传统方法相比，Fluoro-Jade是一种比苏木精和伊红（H&E）或尼氏型染色更敏感和明确的神经元变性标志物，而与抑制银技术相比，它同样敏感，但更简单和可靠。(C) 1997爱思唯尔科学有限公司
Fluoro-Jade is an anionic fluorochrome capable of selectively staining degenerating neurons in brain slices. The histochemical application of Fluoro-Jade results in a simple, sensitive and reliable method for staining degenerating neurons and their processes. The technique will detect neuronal degeneration resulting from exposure to a variety of neurotoxic insults. Fluoro-Jade can be combined with other fluorescent methodologies including immunofluorescence, fluorescent axonal tract tracing, and fluorescent Nissl counterstaining. Compared to conventional methodologies, Fluoro-Jade is a more sensitive and definitive marker of neuronal degeneration than hematoxylin and eosin (H&E) or Nissl type stains, while being comparably sensitive yet considerably simpler and more reliable than suppressed silver techniques. (C) 1997 Elsevier Science B.V.