Construction of a Corneal Stromal Equivalent with SMILE-Derived Lenticules and Fibrin Glue
Construction of a Corneal Stromal Equivalent with SMILE-Derived Lenticules and Fibrin Glue
复制标题
用 SMILE 衍生的微透镜和纤维蛋白胶构建角膜基质等效物。
DOI：
10.1038/srep33848
复制
发表时间：
2016-09-21
影响因子：
4.6
通讯作者：
Yang, Yabo
中科院分区：
文献类型：
--
作者：
Yin, Houfa;Qiu, Peijin;Yang, Yabo
用于治疗深层角膜缺损和角膜穿孔的角膜组织的缺乏仍然是一个挑战。目前，小切口子宫内膜剥脱术（SMILE）衍生的子宫内膜剥脱术似乎是治疗这些疾病的一种有前途的替代方案。然而，单片微珠的厚度和韧性是有限的。为了克服这些局限性，我们构建了一个角膜基质等效与SMILE衍生的明胶和纤维蛋白胶。体外细胞培养结果显示，角膜基质等效物可为角膜上皮细胞的存活和增殖提供合适的支架，形成连续的多层上皮，并表达特征性标志物。最后，兔的前板层角膜移植术证明，具有脱细胞小颗粒和纤维蛋白胶的角膜基质等效物可以修复基质的前部区域，导致再上皮化以及透明度和超微结构组织的恢复。3个月内未观察到角膜新生血管、移植物降解和角膜排斥反应。总之，含SMILE衍生的明胶和纤维蛋白胶的角膜基质等效物似乎是修复前角膜损伤的安全有效的替代方案，这可能为治疗深层角膜缺损或角膜穿孔提供新的途径。
The scarcity of corneal tissue to treat deep corneal defects and corneal perforations remains a challenge. Currently, small incision lenticule extraction (SMILE)-derived lenticules appear to be a promising alternative for the treatment of these conditions. However, the thickness and toughness of a single piece of lenticule are limited. To overcome these limitations, we constructed a corneal stromal equivalent with SMILE-derived lenticules and fibrin glue. In vitro cell culture revealed that the corneal stromal equivalent could provide a suitable scaffold for the survival and proliferation of corneal epithelial cells, which formed a continuous pluristratified epithelium with the expression of characteristic markers. Finally, anterior lamellar keratoplasty in rabbits demonstrated that the corneal stromal equivalent with decellularized lenticules and fibrin glue could repair the anterior region of the stroma, leading to re-epithelialization and recovery of both transparency and ultrastructural organization. Corneal neovascularization, graft degradation, and corneal rejection were not observed within 3 months. Taken together, the corneal stromal equivalent with SMILE-derived lenticules and fibrin glue appears to be a safe and effective alternative for the repair of damage to the anterior cornea, which may provide new avenues in the treatment of deep corneal defects or corneal perforations.