Early Excitatory Activity-Dependent Maturation of Somatostatin Interneurons in Cortical Layer 2/3 of Mice
Early Excitatory Activity-Dependent Maturation of Somatostatin Interneurons in Cortical Layer 2/3 of Mice
复制标题
小鼠皮质层 2/3 中生长抑素中间神经元的早期兴奋活动依赖性成熟。
DOI：
10.1093/cercor/bhy293
复制
发表时间：
2019-10-01
期刊：
影响因子：
3.7
通讯作者：
Wang，Xiaoqun
中科院分区：
文献类型：
--
作者：
Pan，Na Clara;Fang，Ai;Wang，Xiaoqun
GABA能中间神经元在小鼠大脑皮质发育过程中发挥着不同的功能。在大脑中存在的各种GABA能神经元中，早期出生的表达生长抑素(SST)的抑制性中间神经元由其他中间神经元和局部锥体细胞(PC)支配，在电路调节中发挥神经计算作用。SST+中间神经元与其他细胞之间的突触在发育过程中逐渐形成。在此，我们追踪了小鼠新皮质次级运动区(M2)第2/3层SST+中间神经元的电生理特性的发育过程，以及SST+中间神经元与PC之间的突触连接。此外，我们还利用毒素介导和遗传学的方法抑制了神经前体细胞的活性，并证明了在早期(P1之前)而不是晚期(P8之后)减少兴奋性输入会延迟SST+中间神经元的功能成熟。综上所述，我们的结果表明，PC的早期功能活动对SST+中间神经元的内源性成熟至关重要，随后这些中间神经元参与局部环路。
GABAergic interneurons perform distinct functions during cortical development in the mouse brain. Among the diverse GABAergic neurons present in the brain, early-born somatostatin (SST)-expressing inhibitory interneurons, which are innervated by other interneurons and local pyramidal cells (PCs), act in a neural computational role in circuitry regulation. The synapses between the SST+ interneurons and other cells form gradually during development. Here, we traced the developmental course of the electrophysiological properties of SST+ interneurons at layer 2/3 of the neocortical secondary motor area (M2) in mouse, and the synaptic connectivity between SST+ interneurons and PCs. Also, we used toxin-mediated and genetic method to suppress the activities of PCs, and demonstrate that decreasing excitatory input at early stage (before P1) rather than late stage (after P8) would delay the functional maturation of SST+ interneurons. In conclusion, our results indicate that early functional activity of PCs is crucial for the intrinsic maturation of SST+ interneurons, following which these interneurons participate in local circuitry.