THE LIBERATION OF ADENOSINE TRIPHOSPHATE ON ANTIDROMIC STIMULATION OF SENSORY NERVES
THE LIBERATION OF ADENOSINE TRIPHOSPHATE ON ANTIDROMIC STIMULATION OF SENSORY NERVES
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DOI：
10.1113/jphysiol.1959.sp006157
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发表时间：
1959-01-01
影响因子：
5.5
通讯作者：
HOLTON, P
中科院分区：
文献类型：
--
作者：
HOLTON, P
方法灌注法采用Rischbieter（1913）法在40 ~ 200 mm Hg压力下对家兔耳进行灌注，获得合适的灌注液流速（0 ~ 05 ~ 1 ml./min）。在氨基甲酸乙酯麻醉下进行解剖，并注意在建立灌注前尽可能短时间（通常小于2分钟）停止通过耳的血流。在大多数实验中，灌注液为6%葡聚糖（Intradex, Glaxo Ltd.或Dextraven， Benger Laboratories Ltd.），其中加入盐，得到洛克溶液（NaCl 9, KCI 0* 4, CaCl2 0x24, NaHCO3 0.5，葡萄糖1）的组成。0 g/l)，用含5% CO2的氧气平衡后pH为7* 3-7-4。少数实验采用不含右旋糖酐的Locke’s溶液，也得到了类似的结果，但灌流数小时后耳部出现水肿。灌注在室温下进行。静脉流出物被收集在离心管中，周围是碎冰，并保持冷却，直到测试。离心5min，沉淀从组织中冲洗出来的红细胞，上清用于分析。
METHODS Perfusion method Rabbits' ears were perfused by Rischbieter's (1913) method at pressures from 40 to 200 mm Hg to obtain a suitable rate of flow of perfusion fluid (0-05-1 ml./min). The dissection was carried out under urethane anaesthesia and care was taken that the blood flow through the ear was stopped for as short a time as possible, usually less than 2 min, before the perfusion was established. In most experiments the perfusion fluid was 6% dextran (Intradex, Glaxo Ltd. or Dextraven, Benger Laboratories Ltd.) to which salts were added to give the composition of Locke's solution (NaCl 9, KCI 0* 4, CaCl2 0x24, NaHCO3 0.5, glucose1. 0 g/l.) and a pH of 7* 3-7-4 after equilibration with oxygen containing 5% CO2. In a few experiments Locke's solution without dextran was used and similar results were obtained, but the ear became oedematous after a few hours' perfusion.The perfusion was at room temperature. The venous effluent was collected in centrifuge tubes surrounded by crushed ice and was kept cool until it was tested. It was centrifuged for 5 min to sediment the erythrocytes which were washed out of the tissues, and the supernatant was used for analysis.