[The relationship between major histocompatibility complex class I chain-related antigens A (MICA)-129 gene polymorphism, soluble MICA level and ulcerative colitis].
[The relationship between major histocompatibility complex class I chain-related antigens A (MICA)-129 gene polymorphism, soluble MICA level and ulcerative colitis].
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发表时间：
2011-04
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通讯作者：
Jie Zhao;Yi Jiang;Yuan Lei;Liping Chen;Feng-ming Yi;Chang-gao Wang;Kai-fang Zou;B. Xia
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作者：
Jie Zhao;Yi Jiang;Yuan Lei;Liping Chen;Feng-ming Yi;Chang-gao Wang;Kai-fang Zou;B. Xia
目的探讨湖北汉族溃疡性结肠炎（UC）与主要组织相容性复合体I类链相关抗原A（云母）-129基因多态性及可溶性云母（sMICA）水平的关系。方法采用聚合酶链反应-序列基础试验（PCR-SBT）检测256例UC患者和460例健康对照者云母-129基因多态性。从上述受试者中随机抽取80例患者和90例健康人，采用ELISA法测定血清sMICA浓度。结果UC患者云母-129基因变异等位基因（G）和基因型（GG）频率均显著高于对照组（76.8%vs72.2%，P = 0.060; 55.9%vs46.3%，P = 0.016）;患者血清sMICA水平显著高于对照组[（576.47 ± 279.02）ng/L vs（182.17 ± 73.11）ng/L，P < 0.001]。云母-129 GG基因型患者血清sMICA水平高于（GA + AA）基因型患者[（638.87 ± 347.15）ng/L vs（507.51 ± 152.87）ng/L，P = 0.035]。结论云母-129基因多态性和sMICA水平与湖北汉族UC患者相关。我们的研究结果表明，云母-129基因可能有助于UC的发病机制。
OBJECTIVE To investigate the association of the major histocompatibility complex class I chain-related antigens A (MICA)-129 gene polymorphism and soluble MICA (sMICA) levels with ulcerative colitis (UC) in Hubei Han nationality. METHODS The genetic polymorphism of MICA-129 was examined using a polymerase chain reaction-sequence based test (PCR-SBT) in 256 UC patients and 460 healthy controls. From the above subjects, 80 patients and 90 healthy individuals were randomly selected for determining serum sMICA concentrations by ELISA. RESULTS The frequencies of variant allele (G) and genotype (GG) in MICA-129 gene were significantly higher in the UC patients than in the controls (76.8% vs 72.2%, P = 0.060; 55.9% vs 46.3%, P = 0.016). Serum sMICA levels were significantly elevated in the patients compared to the controls [(576.47 ± 279.02) ng/L vs (182.17 ± 73.11) ng/L, P < 0.001]. In addition, the sMICA levels were higher in the patients carrying MICA-129 GG genotypes than in those carrying (GA + AA) genotypes [(638.87 ± 347.15) ng/L vs (507.51 ± 152.87) ng/L, P = 0.035]. CONCLUSIONS The genetic polymorphism of MICA-129 and sMICA levels are correlated with the UC patients in Hubei Han nationality. Our findings demonstrate that MICA-129 gene may contribute to the pathogenesis of UC.