The expression changes of Numblike in rat brain cortex after traumatic brain injury
The expression changes of Numblike in rat brain cortex after traumatic brain injury
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DOI：
10.1007/s10735-011-9383-x
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发表时间：
2012-04
影响因子：
3.2
通讯作者：
Shengyang Jiang;Xiaohong Wu;Yaohua Yan;Jian Xu;Bai Shao;Xun Zhuang;Ying-ying Han;X. Gu
中科院分区：
文献类型：
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作者：
Shengyang Jiang;Xiaohong Wu;Yaohua Yan;Jian Xu;Bai Shao;Xun Zhuang;Ying-ying Han;X. Gu
Numblike（Numbl）在室管膜壁完整性和室下区成神经细胞存活中起重要作用。Numbl在大脑中特异性表达。但其在中枢神经系统病变中的表达及功能尚不清楚。本研究通过建立大鼠创伤性脑损伤（traumatic brain injury，TBI）模型，观察了Numbl在大鼠脑皮层表达的动态变化。Western blot和免疫组化分析显示Numbl在正常脑组织中存在。脑损伤后3d，脑组织中的MDA含量逐渐下降，至伤后3d达到最低点，随后逐渐升高。免疫荧光双标显示Numbl蛋白在神经元中呈阳性反应，而在星形胶质细胞和小胶质细胞中未见阳性反应。损伤后第3天为凋亡高峰，caspase-3表达改变提示凋亡高峰。这些结果提示Numbl可能参与了TBI的病理生理过程，其功能和机制有待进一步研究。
Numblike (Numbl) plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. And Numbl is specifically expressed in the brain. However, its expression and function in the central nervous system lesion are still unclear. In this study, we performed a traumatic brain injury (TBI) model in adult rats and investigated the dynamic changes of Numbl expression in the brain cortex. Western blot and immunohistochemistry analysis revealed that Numbl was present in normal brain. It gradually decreased, reached the lowest point at day 3 after TBI, and then increased during the following days. Double immunofluorescence staining showed that Numbl immunoreactivity was found in neurons, but not astrocytes and microglia. Moreover, the 3rd day post injury was the apoptotic peak implied by the alteration of caspase-3. All these results suggested that Numbl may be involved in the pathophysiology of TBI and further research is needed to have a good understanding of its function and mechanism.