Expression patterns of flagellin sensing 2 map to bacterial entry sites in plant shoots and roots.
Expression patterns of flagellin sensing 2 map to bacterial entry sites in plant shoots and roots.
复制标题
DOI：
10.1093/jxb/eru366
复制
发表时间：
2014-12
影响因子：
6.9
通讯作者：
Robatzek S
中科院分区：
文献类型：
--
作者：
Beck M;Wyrsch I;Strutt J;Wimalasekera R;Webb A;Boller T;Robatzek S
鞭毛蛋白受体FLS 2的表达以与细菌感染部位相关的细胞/组织特异性和应激诱导的方式进行调节。血管系统表达FLS 2并响应鞭毛蛋白。病原体可以在植物的所有器官和组织中定植。为了防止这种情况，每个细胞必须能够自主触发防御。因此，通常认为免疫系统的初级传感器是组成性存在的。针对细菌感染的一种主要的初级传感器是鞭毛蛋白传感2（FLS 2）模式识别受体（PRR）。为了深入了解其表达模式，监测了β-葡萄糖醛酸糖苷酶（GUS）报告细胞系中的FLS 2启动子活性。数据显示，pFLS 2：：GUS活性在易受细菌进入和定殖的细胞和组织中最高，如气孔、排水管和侧根。GUS活性在脉管系统中也很高，并且通过监测脉管系统中的Ca 2+响应，发现该组织有助于flg 22诱导的Ca 2+爆发。FLS 2启动子也以组织和细胞类型特异性的方式调节，并对激素、损伤和生物应激反应。这导致FLS 2表达结构域的刺激依赖性扩增。总之，已经创建了与植物细菌病原体的突出进入位点和靶组织相关的FLS 2表达的组织和细胞类型特异性图谱。
Expression of the flagellin receptor FLS2 is regulated in a cell/tissue-specific and stress-induced manner that correlated with sites of bacterial infection. The vasculature expresses FLS2 and responds to flagellin. Pathogens can colonize all plant organs and tissues. To prevent this, each cell must be capable of autonomously triggering defence. Therefore, it is generally assumed that primary sensors of the immune system are constitutively present. One major primary sensor against bacterial infection is the FLAGELLIN SENSING 2 (FLS2) pattern recognition receptor (PRR). To gain insights into its expression pattern, the FLS2 promoter activity in β-glucuronidase (GUS) reporter lines was monitored. The data show that pFLS2::GUS activity is highest in cells and tissues vulnerable to bacterial entry and colonization, such as stomata, hydathodes, and lateral roots. GUS activity is also high in the vasculature and, by monitoring Ca2+ responses in the vasculature, it was found that this tissue contributes to flg22-induced Ca2+ burst. The FLS2 promoter is also regulated in a tissue- and cell type-specific manner and is responsive to hormones, damage, and biotic stresses. This results in stimulus-dependent expansion of the FLS2 expression domain. In summary, a tissue- and cell type-specific map of FLS2 expression has been created correlating with prominent entry sites and target tissues of plant bacterial pathogens.
登录
查看更多内容
影响因子：
7.7
作者：
Lozano-Durán R;Macho AP;Boutrot F;Segonzac C;Somssich IE;Zipfel C
通讯作者：
Zipfel C
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.0910772107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Alassimone, Julien;Naseer, Sadaf;Geldner, Niko
通讯作者：
Geldner, Niko
影响因子：
3.5
作者：
Hugouvieux, V;Barber, CE;Daniels, MJ
通讯作者：
Daniels, MJ
影响因子：
4.4
作者：
Dong, YM;Iniguez, AL;Triplett, EW
通讯作者：
Triplett, EW