TISSUE-SPECIFICITY OF GLYCOSPHINGOLIPIDS AS EXPRESSED IN PANCREAS AND SMALL-INTESTINE OF BLOOD GROUP-A AND GROUP-B HUMAN INDIVIDUALS
TISSUE-SPECIFICITY OF GLYCOSPHINGOLIPIDS AS EXPRESSED IN PANCREAS AND SMALL-INTESTINE OF BLOOD GROUP-A AND GROUP-B HUMAN INDIVIDUALS
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DOI：
10.1016/0003-9861(84)90048-1
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发表时间：
1984-01-01
影响因子：
3.9
通讯作者：
BREIMER, ME
中科院分区：
文献类型：
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作者：
BREIMER, ME
对 2 个个体（一种血型 A 和一种血型 B）的小肠和胰腺的血型活性鞘糖脂进行了化学研究。制备了总非酸性糖脂级分，并对存在的主要血型岩藻糖脂进行了纯化，并通过质谱、质子核磁共振波谱和降解方法进行了结构表征。鉴定的糖脂结构是血型 Leb 六糖基神经酰胺、具有 1 型 (Gal.beta.1 .fwdarw. 3GlcNAc) 碳水化合物链的 B-六糖基神经酰胺、具有 1 型和 2 型 (Gal.beta.1 .fwdarw. 4GlcNAc) 碳水化合物链的 A-六糖基神经酰胺、具有 1 型碳水化合物链的 B-七糖基神经酰胺、以及具有 1 型和 2 型碳水化合物链的 A-七糖基神经酰胺。通过 TLC 检测到几种具有 > 7 个糖残基的次要糖脂。小肠和胰腺在主要岩藻糖脂的表达方面存在一些明显差异。小肠仅含有基于 1 型碳水化合物链的糖脂，而胰腺同时具有 1 型和 2 型结构。肠主要含有二岩藻糖基化合物，而胰腺组织含有单岩藻糖基糖脂和二岩藻糖基糖脂。一个胰腺中存在基于 1 型和 2 型糖的单岩藻糖基糖脂，而另一个胰腺中仅含有基于 1 型链的单岩藻糖基成分。与胰腺糖脂（二羟基长链碱，非羟基脂肪酸）相比，肠道糖脂的神经酰胺更加羟基化（三羟基长链碱，羟基脂肪酸）。
A chemical investigation was done on blood group active glycosphingolipids of both small intestine and pancreas from 2 individuals, one blood group A and one blood group B. Total non-acid glycolipid fractions were prepared and the major blood group fucolipids present were purified and structurally characterized by mass spectrometry, proton NMR spectroscopy and degradation methods. The glycolipid structures identified were a blood group Leb hexaglycosylceramide, a B-hexaglycosylceramide with a type 1 (Gal.beta.1 .fwdarw. 3GlcNAc) carbohydrate chain, A-hexaglycosylceramides with types 1 and 2 (Gal.beta.1 .fwdarw. 4GlcNAc) carbohydrate chains, a B-heptaglycosylceramide with a type 1 carbohydrate chain, and A-heptaglycosylceramides with type 1 and 2 carbohydrate chains. Several minor glycolipids having > 7 sugar residues were detected by TLC. The small intestine and pancreas had some distinct differences in their expression of the major fucolipids. The small intestine contained only glycolipids based upon type 1 carbohydrate chain while the pancreas had both type 1 and type 2 structures. The intestines contained mainly difucosyl compounds while the pancreas tissues contained both mono- and difucosyl glycolipids. Monofucosylglycolipids based on both types 1 and 2 saccharides were present in one pancreas while the other one contained only monofucosylcomponents based on type 1 chain. The ceramides of the intestinal glycolipids were more hydroxylated (trihydroxy long-chain base, hydroxy fatty acids) compared to the pancreas glycolipids (dihydroxy long-chain base, non-hydroxy fatty acids).