Identification of the pollen determinant of S-RNase-mediated self-incompatibility
Identification of the pollen determinant of S-RNase-mediated self-incompatibility
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DOI：
10.1038/nature02523
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发表时间：
2004-05-20
期刊：
影响因子：
64.8
通讯作者：
Kao, TH
中科院分区：
文献类型：
--
作者：
Sijacic, P;Wang, X;Kao, TH
许多开花植物采用自交不亲和机制来防止近亲繁殖和促进异交(1)。在茄科、蔷薇科和玄参科植物中，控制自交不亲和互作的两个基因位于高度多态的S基因座上：S核糖核酸酶基因编码雌蕊决定基因，S基因编码花粉决定基因(2-4)。S核糖核酸酶与花粉S等位基因产物相互作用，抑制花柱中自交花粉管的生长。最近在蔷薇科和玄参科(5-8)中发现了与S核糖核酸酶基因相近的花粉表达的F-box基因，但到目前为止还没有发现直接编码花粉决定因素的基因。本文报道了茄科矮牵牛的一个S位点F-box基因PiSLF的鉴定和特征分析。我们发现，将S-2等位基因转化到S1S1、S1S2和S2S3植株中，导致了它们的自交不亲和功能的崩溃。花粉功能的这种分解与“竞争相互作用”相一致，即携带两个不同的S等位基因的花粉在自交不亲和(1，9，10)中不起作用。我们得出结论，PiSLF编码花粉自交不亲和决定因素。
Many flowering plants have adopted self-incompatibility mechanisms to prevent inbreeding and promote out-crosses(1). In the Solanaceae, Rosaceae and Scrophulariaceae, two separate genes at the highly polymorphic S-locus control self-incompatibility interactions: the S-RNase gene encodes the pistil determinant and the previously unidentified S-gene encodes the pollen determinant(2-4). S-RNases interact with pollen S-allele products to inhibit the growth of self-pollen tubes in the style. Pollen-expressed F-box genes showing allelic sequence polymorphism have recently been identified near to the S-RNase gene in members of the Rosaceae and Scrophulariaceae(5-8); but until now have not been directly shown to encode the pollen determinant. Here we report the identification and characterization of PiSLF, an S-locus F-box gene of Petunia inflata ( Solanaceae). We show that transformation of S1S1, S1S2 and S2S3 plants with the S-2-allele of PiSLF causes breakdown of their pollen function in self-incompatibility. This breakdown of pollen function is consistent with 'competitive interaction', in which pollen carrying two different pollen S-alleles fails to function in self-incompatibility(1,9,10). We conclude that PiSLF encodes the pollen self-incompatibility determinant.