Identification of Candida dubliniensisin a Prospective Study of Patients in the United States
Identification of Candida dubliniensisin a Prospective Study of Patients in the United States
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在美国患者的前瞻性研究中鉴定都柏林念珠菌
DOI：
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发表时间：
1999
影响因子：
9.4
通讯作者：
T. Meiller
中科院分区：
文献类型：
--
作者：
M. Jabra;A. Baqui;J. Kelley;W. Falkler;W. Merz;T. Meiller
摘要虽然白色念珠菌仍然是最常见的口腔真菌，但在人类免疫缺陷病毒(HIV)血清阳性的患者中也经常发现其他酵母菌。双念珠菌在许多方面与白色念珠菌相似，但通过其表型和遗传特征可以被鉴定和区分为一个独特的念珠菌种。本研究的目的是前瞻性地检测临床分离株中是否存在都柏林螺旋体，并确定携带都柏林螺旋体的患者的临床和人口学特征。在90天的时间里，对来自724名可能被鉴定为白色念珠菌的患者分离的菌株进行了都柏林葡萄球菌的筛选，方法是通过芽管和衣原体孢子产生试验，通过检测在45℃下不能生长，通过在ChROMagar Candida培养基上的菌落颜色，以及通过API 20C AUX酵母鉴定系统的糖同化试验的结果。在这些被评估的标本中检索到的699株分离物中，有5株来自25名HIV血清阳性患者，1株来自HIV状况不明的患者，通过表型分析和与欧洲参考菌株OFC的核型分析显示一致。都柏林。其中一株对氟康唑的敏感性呈剂量依赖性，最低抑菌浓度为16μg/ml。这些结果证实了这种有趣的物种在美国的存在，并支持了进一步研究都柏林隐翅虫的流行和发病机制的必要性。
ABSTRACT Although Candida albicans remains the fungal species most frequently isolated as an opportunistic oral pathogen, other yeast species are often identified in human immunodeficiency virus (HIV)-seropositive patients. Candida dubliniensisphenotypically resembles C. albicans in many respects, yet it can be identified and differentiated as a unique Candidaspecies by its phenotypic and genetic profiles. The purpose of the present study was to prospectively test for the presence of C. dubliniensis among clinical isolates and to determine the clinical and demographic characteristics of patients harboring C. dubliniensis. Over a 90-day period, isolates from 724 patients that were presumptively identified as C. albicans were screened for C. dubliniensis by use of tests for germ tube and chlamydospore production, by detection of an inability to grow at 45°C, by colony color on CHROMagar Candida medium, and by the results of a sugar assimilation test with the API 20C AUX yeast identification system. Among 699 isolates retrieved from those specimens evaluated, 5 from 25 HIV-seropositive patients and 1 isolate from a patient whose HIV status was unknown were shown to be consistent by phenotyping and by electrophoretic karyotyping with the European reference strain ofC. dubliniensis. One of the C. dubliniensisisolates had dose-dependent susceptibility to fluconazole (MIC, 16 μg/ml). These results confirm the presence of this interesting species in the United States and support the need for further investigations into the prevalence and pathogenesis of C. dubliniensis.
影响因子：
158.5
作者：
WINGARD, JR;MERZ, WG;SARAL, R
通讯作者：
SARAL, R