Simultaneous determination of aflatoxins B1, B2, G1, G2 in Fructus Bruceae by high-performance liquid chromatography with online postcolumn photochemical derivatization
Simultaneous determination of aflatoxins B1, B2, G1, G2 in Fructus Bruceae by high-performance liquid chromatography with online postcolumn photochemical derivatization
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DOI：
10.1002/jssc.201400501
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发表时间：
2014-10-01
影响因子：
3.1
通讯作者：
Yang, Shihai
中科院分区：
文献类型：
--
作者：
Cao, Jiliang;Zhou, Shujun;Yang, Shihai
采用高效液相色谱-在线柱后光化学衍生化和荧光检测技术，建立了一种简单、可靠、高灵敏度的同时测定鸦胆子中黄曲霉毒素B-1、B-2、G(1)、G(2)的方法。首先用甲醇/水混合物提取黄曲霉毒素，然后用 AflaTest 免疫亲和柱净化。对不同的净化和衍生化方法进行了比较和优化。所建立的方法经过广泛验证，对所研究的黄曲霉毒素显示出令人满意的线性（R-2 0.9997）、回收率（74.3-100.8%）和精密度（RSD 2.8%）性能。该方法也适用于11个鸦胆子样品，结果显示11个中有10个样品被黄曲霉毒素污染，浓度范围为0.26至27.52 g/kg，其中毒性最强的黄曲霉毒素B-1在所有样品中的出现率高达91%，凸显了鸦胆子污染的严重性和制定法定限量的必要性。
A simple, reliable, and highly sensitive method for the simultaneous determination of aflatoxin B-1, B-2, G(1), G(2) in Fructus Bruceae was developed using high-performance liquid chromatography coupled to online postcolumn photochemical derivatization and fluorescence detection. Aflatoxins were first extracted by a methanol/water mixture and then cleaned up with an AflaTest immunoaffinity column. Different clean-up and derivatization methods were compared and optimized. The established method was extensively validated to show satisfactory performance of linearity (R-2 0.9997), recovery (74.3-100.8%), and precision (RSDs 2.8%) for the investigated aflatoxins. This proposed method was also applied to 11 F. Bruceae samples and the results showed that 10 out of 11 were contaminated with aflatoxins ranging from 0.26 to 27.52 g/kg and the occurrence of aflatoxin B-1, the most toxic one, was as high as 91% in all the samples, highlighting the severe contamination and the necessity to set legal limits for aflatoxins in F. Bruceae.