The rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation
The rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation
复制标题
DOI：
10.1016/s0092-8674(00)80482-1
复制
发表时间：
1997-12-26
期刊：
影响因子：
64.5
通讯作者：
Settleman, J
中科院分区：
文献类型：
--
作者：
Barrett, K;Leptin, M;Settleman, J
Rho GTP酶介导肌动蛋白重排，这可能是发育中胚胎中许多细胞形状变化所需的。在果蝇Rho信号通路组件的遗传筛选中，我们确定了一个基因，DRhoGEF 2，它编码一个预测的Rho特异性鸟嘌呤核苷酸交换因子。缺乏DRhoGEF 2的胚胎由于组织内陷所需的细胞形状变化缺陷而无法原肠胚形成，而早期胚胎中显性阴性Rho GTPase的表达也导致了类似的缺陷。证据还提出，DRhoGEF2介导这些特定的细胞形状的变化，响应细胞外配体，雾。总之，这些结果建立了一个Rho介导的信号通路，是必不可少的果蝇原肠胚形成的主要形态发生事件。
The Rho GTPases mediate actin rearrangements that are likely to be required for the numerous cell shape changes in a developing embryo. In a genetic screen for Rho signaling pathway components in Drosophila, we identified a gene, DRhoGEF2, that encodes a predicted Rho-specific guanine nucleotide exchange factor. Embryos lacking DRhoGEF2 fail to gastrulate due to a defect in cell shape changes required for tissue invagination, and expression of a dominant-negative Rho GTPase in early embryos results in similar defects. Evidence is also presented that DRhoGEF2 mediates these specific cell shape changes in response to the extracellular ligand, Fog. Together, these results establish a Rho-mediated signaling pathway that is essential for the major morphogenetic events in Drosophila gastrulation.