The follicular dendritic cell network in secondary follicles of human palatine tonsils and spleens
The follicular dendritic cell network in secondary follicles of human palatine tonsils and spleens
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DOI：
10.1007/s00418-011-0799-x
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发表时间：
2011-04-01
影响因子：
2.3
通讯作者：
Barth, Peter J.
中科院分区：
文献类型：
--
作者：
Steiniger, Birte;Trabandt, Marc;Barth, Peter J.
人体次级淋巴器官生发中心的结构至今尚未完全研究。我们染色滤泡树突状细胞（FDCs）在人增生性扁桃体和脾脏的连续切片，比较GC的形态学在暴发性免疫反应和静止。对CD 35、CD 21、CD 23和mAb CNA.42靶点的检测证实，完整的人扁桃体GC可由暗区、基底和顶侧亮区以及外区四个区域组成。然而，外区并不是扁桃体GC的恒定特征，在大多数标本中仅存在于少数滤泡中。因此，3 - 60%的扁桃体GC具有CD 23（+）顶端亮区，在个体标本中显示出外区。扁桃体GC中的FDCs似乎对手术期间的机械应力极其敏感。与扁桃体相反，8个成人脾脏中有7个没有表现出不对称的极化GC，而只有对称的GC，没有暗区和亮区或滤泡，GC B细胞很少。一些标本在常规染色后显然只含有初级卵泡，但仔细检查后发现，在FDC中沉积的均匀透明细胞外物质表明存在GC。我们的研究表明，GC的结构在不同的人类次级淋巴器官的变化，最有可能取决于当地的抗原的挑战。
The structure of germinal centres (GCs) in human secondary lymphatic organs has not been thoroughly investigated until now. We stained follicular dendritic cells (FDCs) in serial sections of human hyperplastic tonsils and spleens to compare the morphology of GCs in fulminant immune reactions and quiescence. Detection of CD35, CD21, CD23 and the target of mAb CNA.42 confirmed that full-blown human tonsil GCs may consist of four regions, the dark zone, the basal and apical light zone and the outer zone. The outer zone was, however, not a constant feature of tonsillar GCs and existed only in a minority of follicles in most specimens. Thus, between 3 and 60% of tonsil GCs with a CD23(+) apical light zone exhibited an outer zone in individual specimens. FDCs in tonsil GCs appeared to be extremely sensitive to mechanical stress during surgery. In contrast to tonsils, seven of the eight adult spleens did not exhibit asymmetric polarized GCs, but only symmetric GCs without dark and light zones or follicles with few GC B cells. Some specimens apparently only contained primary follicles after conventional staining, but on closer inspection a homogenous hyaline extracellular material deposited among the FDCs indicated that a GC had been present. Our study demonstrates that the structure of GCs varies in different human secondary lymphatic organs most likely depending on the local antigenic challenge.