Isolation and characterization of hemicelluloses and cellulose from rye straw by alkaline peroxide extraction
Isolation and characterization of hemicelluloses and cellulose from rye straw by alkaline peroxide extraction
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DOI：
10.1023/a:1009245100275
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发表时间：
2000-03-01
期刊：
影响因子：
5.7
通讯作者：
Tomkinson, J
中科院分区：
文献类型：
--
作者：
Fang, JM;Sun, RC;Tomkinson, J
在20℃、30℃、40℃、50℃、60℃、70℃条件下，用2% H2O2在pH为11.5条件下提取水处理过的黑麦秸秆12 h，释放出44.2-71.9%的原始半纤维素和52.7-87.8%的原始木质素。相比之下，在没有H2O2的情况下，用pH为11.5的稀碱性溶液在50℃下处理12 h，只产生7.3%的原始半纤维素和7.4%的原始木质素。木糖是七种可溶性半纤维素制剂中主要的糖成分，少量的阿拉伯糖、葡萄糖和半乳糖也存在。采用傅里叶红外（FT-IR）、碳-13核磁共振（C-13-NMR）、凝胶渗透色谱（GPC）和木质素的硝基苯氧化等方法对半纤维素样品进行了进一步的表征，并报道了结果。最明显的特征是，与从脱木质素的黑麦秸秆中提取的半纤维素相比，在所使用的条件下，秸秆的碱性过氧化处理对半纤维素的整体结构没有影响。
Extraction of water-treated rye straw with 2% H2O2 at pH 11.5 for 12 h at 20 degrees C, 30 degrees C, 40 degrees C, 50 degrees C, 60 degrees C, and 70 degrees C, released 44.2-71.9% of the original hemicelluloses and 52.7-87.8% of the original lignin. As a comparison, treatment of the straw with a dilute alkaline solution at pH 11.5 for 12 h at 50 degrees C, in the absence of H2O2 yielded only 7.3% of the original hemicelluloses and 7.4% of the original lignin. Xylose was the predominant sugar constituent in the seven solubilized hemicellulosic preparations, and arabinose, glucose, and galactose were present in small amounts. The hemicellulosic samples were further characterized by Fourier transform infrared (FT-IR), and carbon-13 magnetic resonance spectroscopy (C-13-NMR), gel permeation chromatography (GPC), and nitrobenzene oxidation of the associated lignin, and the results are reported. The most obvious feature was found that the alkaline peroxide treatment of the straw under the conditions used did not affect the overall structure of the hemicelluloses as compared to the hemicelluloses isolated with alkali from delignified rye straw.