Role of the bilayer in the shape of the isolated erythrocyte membrane.
Role of the bilayer in the shape of the isolated erythrocyte membrane.
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双层在离体红细胞膜形状中的作用。
DOI：
10.1007/bf01872271
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Steck,TL
中科院分区：
文献类型：
--
作者：
Lange,Y;Gough,A;Steck,TL
在对分离的红细胞膜的锯齿状（毛刺状）的研究中探索了细胞形状的决定因素。在5 mmNaPi（pH 8）中制备的标准血影是丰满的、有凹痕的圆盘，即使是由棘细胞（尖刺）红细胞制备的。在等渗盐水、二价阳离子的毫摩尔浓度、1 mm ~ 2、4-二硝基苯酚或0.1mm溶血素存在下，这些血影变成锯齿状。在最小渗透压条件下产生的鬼，在鬼从红细胞部分耗尽胆固醇，和矛盾的是，在鬼从红细胞由溶血素锯齿状抑制。鬼的敏感性，以锯齿状随着时间的推移而消失，这一过程是由升高的温度，低离子强度，和微量的洗涤剂或chlorpromazine.In鬼的形状是由各种amphipaths的影响，我们的研究结果有利于的前提下，双层，而不是subjuncular蛋白质网状驱动鬼锯齿状。这些数据还表明，剧烈的渗透性溶血诱导脂质在双层的两个小叶之间重新分布，这通过双层偶联机制影响膜轮廓。亚稳态分布的随后松弛可以解释所观察到的锯齿状的损失。
The determinants of cell shape were explored in a study of the crenation (spiculation) of the isolated erythrocyte membrane. Standard ghosts prepared in 5mmNaPi(pH 8) were plump, dimpled disks even when prepared from echinocytic (spiculated) red cells. These ghosts became crenated in the presence of isotonic saline, millimolar levels of divalent cations, 1mm2,4-dinitrophenol or 0.1mmlysolecithin. Crenation was suppressed in ghosts generated under conditions of minimal osmotic stress, in ghosts from red cells partially depleted of cholesterol, and, paradoxically, in ghosts from red cells crenated by lysolecithin. The susceptibility of ghosts to crenation was lost with time; this process was potentiated by elevated temperature, low ionic strength, and traces of detergents or chlorpromazine.In that ghost shape was influenced by a variety of amphipaths, our results favor the premise that the bilayer and not the subjacent protein reticulum drives ghost crenation. The data also suggest that vigorous osmotic hemolysis induces a redistribution of lipids between the two leaflets of the bilayer which affects membrane contour through a bilayer couple mechanism. Subsequent relaxation of that metastable distribution could account for the observed loss of crenatability.