Gene expression analysis of CCNs along with odontoblastic differentiation in vivo.
Gene expression analysis of CCNs along with odontoblastic differentiation in vivo.
复制标题
CCN 的基因表达分析以及体内成牙本质细胞分化。
DOI：
10.1007/978-1-0716-2744-0_19
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kubota S.
中科院分区：
文献类型：
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作者：
Kawata K;Kubota S.
牙髓细胞（DPC）分化为成牙本质细胞。检测牙本质形成特异性分子如牙本质涎磷蛋白（DSPP）和碱性磷酸酶（ALP）活性是观察成牙本质细胞分化的可靠方法。CCN家族成员2（CCN 2）已被提议作为牙本质形成的标志物。我们最近的研究表明，Ccn 4，Ccn 5和Ccn 6的表达水平随着成牙本质细胞分化而改变。因此，Ccn 4、Ccn 5和Ccn 6以及Ccn 2可以作为一套完整的牙本质发生标记。在这里，我们描述了一种方法来测量Ccns表达水平在分化大鼠DPC。
Dental pulp cells (DPCs) differentiate into odontoblasts. To observe odontoblastic differentiation, the detection of dentinogenesis-specific molecules such as dentin sialophosphoprotein (DSPP) and the measurement of alkaline phosphatase (ALP) activity are reliable approaches. CCN family member 2 (CCN2) has been proposed as a marker for dentinogenesis. Our recent study revealed that the expression levels ofCcn4,Ccn5, andCcn6were changed in accordance with odontoblastic differentiation. Therefore,Ccn4,Ccn5, andCcn6, as well asCcn2, could serve as a comprehensive set of markers for dentinogenesis. Here, we describe a method of measuring theCcnsexpression levels in differentiating rat DPCs.