Development of wild barley-derived DArT markers and their integration into a barley consensus map
Development of wild barley-derived DArT markers and their integration into a barley consensus map
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DOI：
10.1007/s11032-010-9415-3
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Steffenson, B. J.
中科院分区：
文献类型：
--
作者：
Alsop, B. P.;Farre, A.;Steffenson, B. J.
开发野生大麦特异性基因组文库的目的是创建一个“全面”的原始Hordeum基因库基因组表示，能够对大麦进行更强大的基因分型。为了丰富DArT文库中野生大麦特异性序列，以栽培大麦为减法驱动基因，野生大麦为测试基因，进行了抑制减法杂交（SSH）。4个双单倍体群体，其中2个来自野生×栽培杂交（Damon/Harrington和Shechem/Harrington）， 2个来自栽培×栽培杂交（Albacete/Barbarrouse和TX9425/Naso Nijo）。基因分型数据分析显示，在DArT标记开发的应用中，SSH过程在富集独特序列方面有些无效。然而，添加来自野生大麦的标记被证明是一种有效的方法，可以增加从单次DArT试验中获得的多态性标记的数量。建立了4个组成群体的遗传图谱，并将607个新开发的DArT标记与大麦共识图谱进行整合，构建了包含3542个标记的大麦基因组合成图谱。这大大提高了共识图谱的分辨率，并提高了图谱为描述Hordeum主要基因库的遗传多样性提供参考的能力。综合DArT基因组表示的基因分型能力的提高和合成图谱的更高分辨率，使DArT标记具有更大的灵活性，可作为基于分子标记的大麦育种的快速、高通量平台。
Wild barley-specific genomic libraries were developed for the purpose of creating a 'comprehensive' genomic representation of the primary Hordeum genepool capable of more robust genotyping of barley. In order to enrich for wild barley-specific sequences in the DArT libraries, suppression subtraction hybridization (SSH) was performed using cultivated barley as the subtraction driver and wild barley as the tester. Four doubled-haploid populations were genotyped with the comprehensive barley DArT array, including two from wild x cultivated crosses (Damon/Harrington and Shechem/Harrington) and two from cultivated x cultivated crosses (Albacete/Barbarrouse and TX9425/Naso Nijo). Analysis of genotyping data revealed that the SSH process was somewhat ineffective at enriching for unique sequences in this application of DArT marker development. However, the addition of markers derived from wild barley proved to be an effective means for increasing the number of polymorphic markers obtainable from a single DArT assay. Genetic maps of the four component populations were developed and 607 newly developed DArT markers were integrated with a barley consensus map to create a new synthetic map of the barley genome containing 3542 markers. This significantly increased the resolution of the consensus map and improved the power of the map to provide a reference for profiling genetic diversity within the primary Hordeum genepool. The improvement in the genotyping capability of the comprehensive DArT genomic representation and the higher resolution of the synthetic map facilitates an even greater flexibility of DArT markers to be utilized as a fast, high-throughput platform for molecular marker-based barley breeding.