Parvovirus B19-induced vascular damage in the heart is associated with elevated circulating endothelial microparticles
Parvovirus B19-induced vascular damage in the heart is associated with elevated circulating endothelial microparticles
复制标题
DOI：
10.1371/journal.pone.0176311
复制
发表时间：
2017-05-22
期刊：
影响因子：
3.7
通讯作者：
Boehm, Michael
中科院分区：
文献类型：
--
作者：
Bachelier, Katrin;Biehl, Susanne;Boehm, Michael
背景病毒性心肌炎的诊断很难通过临床标准，但通过检测心肌内膜活检中的炎症和病毒基因组可以很容易地诊断。细小病毒B19（B19 V）靶向内皮细胞，其中病毒核酸仅在心脏中检测到。微粒（MP）在细胞损伤或特定细胞活化后释放。目的探讨循环内皮细胞MP（circulating endothelial MPs，EMPs）与B19 V心肌炎的关系。方法将54例心肌炎患者分为B19 V阳性组（23例）和B19 V阴性组（31例），并与健康对照组（HCTR，n = 25）进行比较。在B19 V转基因小鼠（B19 V-NS 1+）和柯萨奇病毒B3（CVB 3）感染的小鼠中也研究了MP。结果B19 V+与B19 V-、HCTR相比，EMP亚群分布有显著性差异（P
BackgroundDiagnosis of viral myocarditis is difficult by clinical criteria but facilitated by detection of inflammation and viral genomes in endomyocardial biopsies. Parvovirus B19 (B19V) targets endothelial cells where viral nucleic acid is exclusively detected in the heart. Microparticles (MPs) are released after cell damage or activation of specific cells. We aimed to investigate whether circulating endothelial MPs (EMPs) in human and experimental models of myocarditis are associated with B19V myocarditis.MethodsMPs were investigated in patients with myocarditis (n = 54), divided into two groups: B19V+ (n = 23) and B19V- (n = 31) and compared with healthy controls (HCTR, n = 25). MPs were also investigated in B19V transgenic mice (B19V-NS1+) and mice infected with coxsackievirus B3 (CVB3). MPs were analyzed with fluorescent activated cell sorting (FACS).ResultsIn human samples, EMP subpopulation patterns were significantly different in B19V+ compared to B19V-and HCTR (p