Decreased numbers and impaired function of circulating dendritic cell subsets in patients with chronic hepatitis B infection (R2)
Decreased numbers and impaired function of circulating dendritic cell subsets in patients with chronic hepatitis B infection (R2)
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DOI：
10.1111/j.1440-1746.2004.03529.x
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发表时间：
2005-02-01
影响因子：
4.1
通讯作者：
Wang, FS
中科院分区：
文献类型：
--
作者：
Duan, XZ;Zhuang, H;Wang, FS
背景和目的：为探讨B型肝炎病毒（HBV）感染患者外周血树突状细胞（DC）亚群的频率、数量和功能，采用体外细胞培养方法，检测了HBV感染不同阶段患者外周血DC前体细胞亚群（包括pDC 1和pDC 2）及其功能。采用体外培养的方法，检测慢性B型肝炎（CH B）患者外周血单个核细胞（PBMC）DC 1的共刺激分子表达及同种异体刺激混合淋巴细胞反应（AMLR）。结果：与正常对照组相比，肝硬化（LC）患者外周血单个核细胞（PBMC）中pDC 1的数量明显减少（P < 0. 05），而正常对照组PBMC中pDC 1的数量明显减少（P < 0. 05）。然而，pDC 2数量在CHB和LC患者中均减少。CHB患者DC I共刺激分子CD 80、CD 86表达较正常对照组低，同种异体刺激混合淋巴细胞反应（AMLR）受损。PBMC分泌IFN-α的能力。结论：慢性HBV感染者外周血中pDC 1共刺激分子的表达明显降低，AMLR受损，pDC 2数量减少，功能受损，这可能与慢性HBV感染者的病情进展有关。(C)2004 Blackwell Publishing Asia Pry Ltd.
Background and Aim: To investigate the frequencies, numbers and function of circulating dendritic cell (DC) subsets in patients with hepatitis B virus (HBV) infection, we assayed the circulating precursor DC subsets (including pDC1 and pDC2) and their ability in patients at various stages of HBV infection in vitro.Methods: Circulating pDC1 and pDC2 frequencies in peripheral blood mononuclear cells (PBMC) were analyzed by flow cytometric analysis. Costimulatory molecule expression and allostimulatory mixed lymphocyte reaction (AMLR) of DC1, cultured from PBMC in vitro, were detected in patients with chronic hepatitis B (CHB). On behalf of pDC2, interferon (IFN)-alpha production of PBMC was determined by the ELISA method in HBV-infected patients.Results: The number of circulating pDC1 decreased only in patients with liver cirrhosis (LC) compared with that in normal controls. However, pDC2 numbers decreased in both CHB and LC patients. DC I from CHB patients showed lower expression of costimulatory molecules CD80, CD86 and impaired allostimulatory mixed lymphocyte reaction (AMLR) compared with those in normal controls. The ability of PBMC to secrete IFN-alpha. also decreased significantly in patients with chronic HBV infection.Conclusions: Our results suggest that patients with chronic HBV infection have a significantly lower expression of costimulatory molecules and impaired AMLR of pDC1, as well as decreased number and impaired function of circulating pDC2, which may be partially related to HBV disease progression in these patients. (C) 2004 Blackwell Publishing Asia Pry Ltd.