The slug gene is not essential for mesoderm or neural crest development in mice
The slug gene is not essential for mesoderm or neural crest development in mice
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DOI：
10.1006/dbio.1998.8909
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发表时间：
1998-06-15
影响因子：
2.7
通讯作者：
Gridley, T
中科院分区：
文献类型：
--
作者：
Jiang, RL;Lan, Y;Gridley, T
Slug基因编码一种锌指蛋白，与果蝇蜗牛基因的产物同源，与几种脊椎动物中胚层细胞和神经脊细胞的发生和迁移有关。本文描述了小鼠鼻涕虫(Slugh)基因的克隆和遗传分析。Slugh编码一个由269个氨基酸组成的蛋白质，与鸡的鼻涕虫基因产物有92%的氨基酸同源性。我们通过Singh mRNA的整体原位杂交和通过同源重组产生的框内Slugh(LacZ)等位基因检测P-半乳糖苷酶的表达，表征了Slugh基因在小鼠早期胚胎发育过程中的表达。Singh的表达首先在胚外中胚层中检测到，后来在许多中胚层亚群中检测到，尽管在原始条纹中没有检测到它。与许多其他脊椎动物不同，小鼠的鼻涕虫基因在迁移前的神经脊细胞中不表达，但在迁移的神经脊细胞中表达。对缺失所有Singh编码序列的靶向零突变的分析表明，Slugh对于小鼠中胚层的形成或神经脊的生成、迁移或发育都不是必需的。这些结果表明，在所有脊椎动物中，Slug基因的表达模式和生物学功能都不是保守的。这些数据还提出了一些有趣的问题，即脊椎动物亚门的显著特征之一--神经脊的产生。(C)1998年学术出版社。
The Slug gene encodes a zinc finger protein, homologous to the product of the Drosophila Snail gene, that is implicated in the generation and migration of both mesoderm and neural crest cells in several vertebrate species. We describe here the cloning and genetic analysis of the mouse Slug (Slugh) gene. Slugh encodes a 269-amino-acid protein that shares 92% amino acid identity with the product of the chicken Slug gene. We have characterized Slugh gene expression during early mouse embryogenesis by whole mount in situ hybridization of Singh mRNA and through detection of P-galactosidase expression from an in-frame Slugh(lacZ) allele generated through homologous recombination. Singh expression is first detected in extraembryonic mesoderm and is later detected in many mesodermal subsets, although it is not detected in the primitive streak. In contrast to many other vertebrates, the mouse Slug gene is not expressed in premigratory neural crest cells but is expressed in migratory neural crest cells. Analysis of a targeted null mutation that deleted all Singh coding sequences revealed that Slugh is not required for mesoderm formation or for neural crest generation, migration, or development in mice. These results indicate that neither the expression pattern nor the biological function of the Slug gene is conserved among all vertebrates. These data also raise interesting questions about the regulation of neural crest generation, which is one of the distinguishing characteristics of the vertebrate subphylum. (C) 1998 Academic Press.