AT LEAST 2 NON-ANTIGEN-BINDING MOLECULES ARE REQUIRED FOR SIGNAL TRANSDUCTION BY THE T-CELL ANTIGEN RECEPTOR
AT LEAST 2 NON-ANTIGEN-BINDING MOLECULES ARE REQUIRED FOR SIGNAL TRANSDUCTION BY THE T-CELL ANTIGEN RECEPTOR
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DOI：
10.1073/pnas.85.22.8613
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
WEISS, A
中科院分区：
文献类型：
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作者：
GOLDSMITH, MA;DAZIN, PF;WEISS, A
在T细胞体细胞突变体J.CaM1中，T细胞抗原受体复合物与肌醇磷脂第二信使系统偶联较差;一些针对该受体的不变CD 3亚基的抗体在J.CaM1中保留其激动剂功能。在这里，我们显示的组合互补试验，在J. CaM 1的突变影响的分子，然后抗原结合Ti亚基表明，Ti是间接耦合到信号转导装置通过一个途径，涉及的CD 3复合物。我们还描述了另一种突变体，J.CaM2，其中受体复合物解偶联肌醇磷脂水解。J.CaM2定义了一个额外的互补基团，表明抗原受体的信号转导依赖于至少两个不同于Ti的分子。
In the T-cell somatic mutant J.CaM1, the T-cell antigen receptor complex is poorly coupled to the inositolophospholipid second messenger system; some antibodies against the invariant CD3 subunit of the receptor retain their agonist function in J.CaMl. Here we show by a combination of complementation assays that the mutation in J. CaM1 affects a molecule other then the antigen-binding Ti subunit suggesting that Ti is coupled indirectly to the signal transduction apparatus through a pathway involving the CD3 complex. We also describe another mutant, J.CaM2, in which the receptor complex uncoupled from inositolphospholipid hydrolysis. J.CaM2 defines an additional complementation group, suggesting that signal transduction by the antigen receptor depends on at least two molecules distinct from Ti.