Genome-wide analysis of CrRLK1L gene family in Gossypium and identification of candidate CrRLK1L genes related to fiber development
Genome-wide analysis of CrRLK1L gene family in Gossypium and identification of candidate CrRLK1L genes related to fiber development
复制标题
棉花 CrRLK1L 基因家族的表征以及与纤维发育相关的候选 CrRLK1L 基因的鉴定
DOI：
10.1007/s00438-016-1169-0
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发表时间：
2016-06-01
影响因子：
3.1
通讯作者：
Guo, Wangzhen
中科院分区：
文献类型：
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作者：
Niu, Erli;Cai, Caiping;Guo, Wangzhen
CrRLK1L家族的成员是受体样激酶(RLK)基因家族的一个亚群，被认为是细胞壁完整性的传感器和极性伸长的调节器。为了更好地了解CrRLK1L家族在纤维发育中的各种功能，我们进行了棉花CrRLK1L家族的全基因组鉴定和特征分析。从三个棉种中分别鉴定出44、40和79个CrRLK1L基因：二倍体陆地棉(D-5)、二倍体亚洲棉(A(2))和四倍体陆地棉TM-1(AD(1))。Raimondii的44个CrRLK1L不均匀地定位在12条染色体上，分为6个群(I-VI)，其中群II和群IV又分为两个亚群(分别为群IIa和群IIb，群IVa和群IVb)。这些CRRLK1基因在棉花中表现出高度规律性的发育和空间调控模式。利用5个染色体片段导入系(CSIL)的转录组数据和CrRLK1L与纤维品质性状相关的数量性状基因(QTL)的物理整合，揭示了6个CrRLK1L基因与纤维发育高度相关。本研究为棉花CRRLK1基因的全基因组整合鉴定提供了新的思路，为棉纤维遗传改良提供了参考。
Members of the CrRLK1L family, a subgroup of the receptor-like kinase (RLK) gene family, are thought to act as sensors for the integrity of the cell wall and regulators of polar elongation. To better understand the various functions in fiber development, we conducted genome-wide identification and characterization analyses of CrRLK1L family in cotton. Here 44, 40, and 79 CrRLK1L genes were identified from three cotton species: diploid G. raimondii (D-5), diploid G. arboreum (A(2)), and tetraploid G. hirsutum TM-1 (AD(1)), respectively. The 44 CrRLK1Ls in G. raimondii were anchored to the 12 chromosomes unevenly and were classified into six groups (I-VI), with group II and group IV being further divided into two subgroups (groups IIa and IIb, and IVa and IVb, respectively). These CrRLK1Ls displayed a highly regular pattern of developmental and spatial regulation in cotton. Using the transcriptome data of five chromosomal segment introgression lines (CSILs) and the physical integration of CrRLK1Ls with the quantitative trait loci (QTLs) related to fiber quality traits, we revealed that six CrRLK1L genes were highly associated with fiber development. This study brings new insights into the integrated genome-wide identification of CrRLK1Ls in cotton and provides references for the genetic improvement of cotton fiber.